Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7297 | 3' | -56 | NC_001900.1 | + | 13011 | 0.67 | 0.614577 |
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Target: 5'- aCgUGGUCaGCGGC-GGCGGcgagGGcuGAGCc -3' miRNA: 3'- -GgACUAG-CGCCGaCCGCCaa--CC--UUCG- -5' |
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| 7297 | 3' | -56 | NC_001900.1 | + | 18105 | 0.7 | 0.446424 |
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Target: 5'- uCCUGAUCgGUGGCaucucccGGCuGGUUGGcguccuGGCa -3' miRNA: 3'- -GGACUAG-CGCCGa------CCG-CCAACCu-----UCG- -5' |
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| 7297 | 3' | -56 | NC_001900.1 | + | 6626 | 0.7 | 0.436712 |
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Target: 5'- --gGGUCGaaCGGCUGGCuguaggccGGUUGGAuGCc -3' miRNA: 3'- ggaCUAGC--GCCGACCG--------CCAACCUuCG- -5' |
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| 7297 | 3' | -56 | NC_001900.1 | + | 1143 | 0.7 | 0.417659 |
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Target: 5'- gCCgGGgcagGCGGCgugGGUGGacUUGGAGGCg -3' miRNA: 3'- -GGaCUag--CGCCGa--CCGCC--AACCUUCG- -5' |
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| 7297 | 3' | -56 | NC_001900.1 | + | 24825 | 0.71 | 0.390052 |
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Target: 5'- gCUcAUCGCuGCcGGUGGUgcUGGAGGCa -3' miRNA: 3'- gGAcUAGCGcCGaCCGCCA--ACCUUCG- -5' |
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| 7297 | 3' | -56 | NC_001900.1 | + | 45230 | 0.71 | 0.372323 |
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Target: 5'- gCUGGUCGa-GCgucacgGGUGGcUGGAAGCa -3' miRNA: 3'- gGACUAGCgcCGa-----CCGCCaACCUUCG- -5' |
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| 7297 | 3' | -56 | NC_001900.1 | + | 874 | 0.72 | 0.338542 |
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Target: 5'- gUCUGGggUCGCccaGCacGCGGUUGGGAGCa -3' miRNA: 3'- -GGACU--AGCGc--CGacCGCCAACCUUCG- -5' |
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| 7297 | 3' | -56 | NC_001900.1 | + | 13170 | 1.14 | 0.00035 |
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Target: 5'- gCCUGAUCGCGGCUGGCGGUUGGAAGCg -3' miRNA: 3'- -GGACUAGCGCCGACCGCCAACCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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