Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7298 | 3' | -56.8 | NC_001900.1 | + | 12070 | 0.66 | 0.585466 |
Target: 5'- --uUCAUGGCGUUGAggaucgggucaccgUUGAGCggcaguuccuGCCGAc -3' miRNA: 3'- uucAGUACCGCGACU--------------AGCUCG----------CGGCU- -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 3914 | 0.66 | 0.577744 |
Target: 5'- cGAGuUCGUcGGCgaGCUGGcCGAGaCGCCGGu -3' miRNA: 3'- -UUC-AGUA-CCG--CGACUaGCUC-GCGGCU- -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 34167 | 0.66 | 0.53414 |
Target: 5'- gGGGagAUGGcCGCUGAggucgacaGAGCGgCCGAg -3' miRNA: 3'- -UUCagUACC-GCGACUag------CUCGC-GGCU- -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 31308 | 0.67 | 0.471152 |
Target: 5'- --aUCGUGGCGUUG-UCG-GUGCCGc -3' miRNA: 3'- uucAGUACCGCGACuAGCuCGCGGCu -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 15094 | 0.68 | 0.43717 |
Target: 5'- -cGUCGaccUGGacuggagccaccgGCUGGUCGAGCGUCGGc -3' miRNA: 3'- uuCAGU---ACCg------------CGACUAGCUCGCGGCU- -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 23820 | 0.68 | 0.43132 |
Target: 5'- cGGUCGgcuaucGGCGCUGGcuUCcGGCGCUGGg -3' miRNA: 3'- uUCAGUa-----CCGCGACU--AGcUCGCGGCU- -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 29434 | 0.68 | 0.421672 |
Target: 5'- uGGGUCc-GGUGCUGAUCG-GCaCCGAc -3' miRNA: 3'- -UUCAGuaCCGCGACUAGCuCGcGGCU- -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 13403 | 0.69 | 0.412156 |
Target: 5'- cGAGUC--GGCGCUcGAUC-AGCGCCa- -3' miRNA: 3'- -UUCAGuaCCGCGA-CUAGcUCGCGGcu -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 35727 | 0.7 | 0.332785 |
Target: 5'- aGAGgcaCAUGGCGCUGGUUccggggcaagaaGGGCGUCGu -3' miRNA: 3'- -UUCa--GUACCGCGACUAG------------CUCGCGGCu -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 43928 | 0.72 | 0.258439 |
Target: 5'- -cGUCccGGUGCUGAgaUCGAGCGUCu- -3' miRNA: 3'- uuCAGuaCCGCGACU--AGCUCGCGGcu -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 22486 | 0.75 | 0.172972 |
Target: 5'- ---aCAUgaGGCGCUGAacaUCGAGCGCCGc -3' miRNA: 3'- uucaGUA--CCGCGACU---AGCUCGCGGCu -5' |
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7298 | 3' | -56.8 | NC_001900.1 | + | 13325 | 1.06 | 0.000819 |
Target: 5'- gAAGUCAUGGCGCUGAUCGAGCGCCGAc -3' miRNA: 3'- -UUCAGUACCGCGACUAGCUCGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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