Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7299 | 3' | -54.1 | NC_001900.1 | + | 23477 | 0.66 | 0.719385 |
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Target: 5'- uGUggCUCCcUGGuCGGUCcACucgGCCUGAUg -3' miRNA: 3'- cCAaaGAGG-ACC-GCCAG-UG---UGGACUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 15548 | 0.66 | 0.719385 |
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Target: 5'- cGGccUCUCgCcgGGCGGUgGaGCCUGACc -3' miRNA: 3'- -CCaaAGAG-Ga-CCGCCAgUgUGGACUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 4123 | 0.67 | 0.697535 |
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Target: 5'- -----aUCgaGGCGGUCACGCCgucGAUc -3' miRNA: 3'- ccaaagAGgaCCGCCAGUGUGGa--CUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 18112 | 0.67 | 0.686506 |
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Target: 5'- cGGUggcaUCUCCcGGCuGGUUgGCGuCCUGGCa -3' miRNA: 3'- -CCAa---AGAGGaCCG-CCAG-UGU-GGACUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 33015 | 0.68 | 0.619595 |
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Target: 5'- cGGUgUCUCCaaGGCGGggaACACCU-ACg -3' miRNA: 3'- -CCAaAGAGGa-CCGCCag-UGUGGAcUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 31596 | 0.68 | 0.59727 |
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Target: 5'- cGGUgagCUUCUGGCGGUUcgGCCgguucgGGCc -3' miRNA: 3'- -CCAaa-GAGGACCGCCAGugUGGa-----CUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 24691 | 0.68 | 0.59727 |
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Target: 5'- ---aUC-CCgugGGCGGUCACACagaucaCUGGCg -3' miRNA: 3'- ccaaAGaGGa--CCGCCAGUGUG------GACUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 20947 | 0.72 | 0.38137 |
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Target: 5'- gGGUUcgUCC-GGCGGUCGucaACCUGGCu -3' miRNA: 3'- -CCAAagAGGaCCGCCAGUg--UGGACUG- -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 156 | 0.74 | 0.31377 |
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Target: 5'- cGUggCUCCUGGUGGcUCACagcGCCUGGg -3' miRNA: 3'- cCAaaGAGGACCGCC-AGUG---UGGACUg -5' |
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| 7299 | 3' | -54.1 | NC_001900.1 | + | 13684 | 1.11 | 0.00086 |
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Target: 5'- gGGUUUCUCCUGGCGGUCACACCUGACc -3' miRNA: 3'- -CCAAAGAGGACCGCCAGUGUGGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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