miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
730 5' -46 AC_000020.1 + 25348 0.66 0.973286
Target:  5'- gGCGGCGAuGAAGA----GCUGAAGc -3'
miRNA:   3'- aCGUCGCUuCUUCUagguUGAUUUCu -5'
730 5' -46 AC_000020.1 + 24791 0.67 0.948162
Target:  5'- cGCGGCGucGAGGAUgCCuguuGCagggUGAGGAa -3'
miRNA:   3'- aCGUCGCuuCUUCUA-GGu---UG----AUUUCU- -5'
730 5' -46 AC_000020.1 + 27015 0.68 0.910901
Target:  5'- uUGUAGCGggGGAGGgg-GAgUAGGGAa -3'
miRNA:   3'- -ACGUCGCuuCUUCUaggUUgAUUUCU- -5'
730 5' -46 AC_000020.1 + 23668 0.7 0.842259
Target:  5'- gGCAGCGAGGAGGA-CCuag-AAAGc -3'
miRNA:   3'- aCGUCGCUUCUUCUaGGuugaUUUCu -5'
730 5' -46 AC_000020.1 + 5534 1.11 0.003388
Target:  5'- uUGCAGCGAAGAAGAUCCAACUAAAGAc -3'
miRNA:   3'- -ACGUCGCUUCUUCUAGGUUGAUUUCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.