Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7300 | 5' | -53.1 | NC_001900.1 | + | 9768 | 0.66 | 0.834978 |
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Target: 5'- -aCGUCUCGUu-GGGGGuguagaGCGUGGc -3' miRNA: 3'- ugGCAGAGCAuuCCUCCug----UGUACCc -5' |
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| 7300 | 5' | -53.1 | NC_001900.1 | + | 42963 | 0.67 | 0.767044 |
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Target: 5'- gACCGcCUcCGUcGAGGGGGACGCGa--- -3' miRNA: 3'- -UGGCaGA-GCA-UUCCUCCUGUGUaccc -5' |
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| 7300 | 5' | -53.1 | NC_001900.1 | + | 20359 | 0.67 | 0.746177 |
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Target: 5'- uACCGggUgGUcAAGGAGGACuCcgGGGa -3' miRNA: 3'- -UGGCagAgCA-UUCCUCCUGuGuaCCC- -5' |
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| 7300 | 5' | -53.1 | NC_001900.1 | + | 42064 | 0.68 | 0.681107 |
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Target: 5'- cGCCGUCgUUGUAcGGGccGGugACGUGGu -3' miRNA: 3'- -UGGCAG-AGCAUuCCU--CCugUGUACCc -5' |
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| 7300 | 5' | -53.1 | NC_001900.1 | + | 31648 | 0.69 | 0.625444 |
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Target: 5'- cACCGag-CGUGAGGuccGGGACAUccGGGa -3' miRNA: 3'- -UGGCagaGCAUUCC---UCCUGUGuaCCC- -5' |
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| 7300 | 5' | -53.1 | NC_001900.1 | + | 14523 | 1.11 | 0.00113 |
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Target: 5'- cACCGUCUCGUAAGGAGGACACAUGGGg -3' miRNA: 3'- -UGGCAGAGCAUUCCUCCUGUGUACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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