Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7306 | 5' | -54.3 | NC_001900.1 | + | 37633 | 0.67 | 0.588006 |
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Target: 5'- cGcGACGGUUUCAAGCucgaccacgccaAGaUCUCCGAGg -3' miRNA: 3'- aC-CUGUCGAAGUUCG------------UCgAGAGGCUU- -5' |
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| 7306 | 5' | -54.3 | NC_001900.1 | + | 23074 | 0.67 | 0.588006 |
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Target: 5'- gGGACAccGCgagcCAGGUAGC-CUCCGGg -3' miRNA: 3'- aCCUGU--CGaa--GUUCGUCGaGAGGCUu -5' |
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| 7306 | 5' | -54.3 | NC_001900.1 | + | 8626 | 0.67 | 0.588006 |
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Target: 5'- cUGGACGGCUcuggUC-GGCuGCcgggUCUCCGAc -3' miRNA: 3'- -ACCUGUCGA----AGuUCGuCG----AGAGGCUu -5' |
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| 7306 | 5' | -54.3 | NC_001900.1 | + | 22778 | 0.68 | 0.565342 |
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Target: 5'- aGaGGCAGCUUCAgcAGguGCUCg-CGGAg -3' miRNA: 3'- aC-CUGUCGAAGU--UCguCGAGagGCUU- -5' |
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| 7306 | 5' | -54.3 | NC_001900.1 | + | 47687 | 0.68 | 0.554093 |
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Target: 5'- aGGGCAGCcUCAcgcucuGCAGCgaUCUCgGAGg -3' miRNA: 3'- aCCUGUCGaAGUu-----CGUCG--AGAGgCUU- -5' |
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| 7306 | 5' | -54.3 | NC_001900.1 | + | 17004 | 0.71 | 0.397218 |
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Target: 5'- aUGGACGGCaUCAAgGCGGCag-CCGAGa -3' miRNA: 3'- -ACCUGUCGaAGUU-CGUCGagaGGCUU- -5' |
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| 7306 | 5' | -54.3 | NC_001900.1 | + | 31596 | 0.74 | 0.231402 |
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Target: 5'- cGGugAGCUUCuGGCGGUUCggCCGGu -3' miRNA: 3'- aCCugUCGAAGuUCGUCGAGa-GGCUu -5' |
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| 7306 | 5' | -54.3 | NC_001900.1 | + | 17808 | 1.06 | 0.001165 |
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Target: 5'- gUGGACAGCUUCAAGCAGCUCUCCGAAa -3' miRNA: 3'- -ACCUGUCGAAGUUCGUCGAGAGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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