Results 1 - 20 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7308 | 3' | -51.3 | NC_001900.1 | + | 23009 | 0.66 | 0.901512 |
Target: 5'- aCGGUCuccAGGGCgCucgucAGACCGuugACCAGa -3' miRNA: 3'- aGCCAG---UUCUG-Gu----UCUGGCua-UGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 34021 | 0.66 | 0.89867 |
Target: 5'- cCuGUCGGGGCCAccgugcgcuucuuGACCGGcACCGGg -3' miRNA: 3'- aGcCAGUUCUGGUu------------CUGGCUaUGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 18335 | 0.66 | 0.894322 |
Target: 5'- gCGGUgagCAAGGCCAAGgaACUG--GCCAGc -3' miRNA: 3'- aGCCA---GUUCUGGUUC--UGGCuaUGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 21381 | 0.66 | 0.894322 |
Target: 5'- gCGGguUCAGGACCGcauccaucAGACCGc--CCAGc -3' miRNA: 3'- aGCC--AGUUCUGGU--------UCUGGCuauGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 44918 | 0.66 | 0.894322 |
Target: 5'- aCGGUgGAGACCGcguuGACCGGcaaCGGc -3' miRNA: 3'- aGCCAgUUCUGGUu---CUGGCUaugGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 35765 | 0.66 | 0.889872 |
Target: 5'- -aGGUCAucguccagcgcgacgAGACCAAGuACCcc-GCCAGa -3' miRNA: 3'- agCCAGU---------------UCUGGUUC-UGGcuaUGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 13934 | 0.66 | 0.886849 |
Target: 5'- uUCGGgguAGACgAGcaccGGCUGGUACCGGg -3' miRNA: 3'- -AGCCaguUCUGgUU----CUGGCUAUGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 23316 | 0.66 | 0.886849 |
Target: 5'- gCGGUCGgccccggcuGGGCCGAGuuCuAUACCGGc -3' miRNA: 3'- aGCCAGU---------UCUGGUUCugGcUAUGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 3373 | 0.66 | 0.886849 |
Target: 5'- -gGGUCGAggugggggaucGACCAGcggaagucgcGGCCGGUgACCAGg -3' miRNA: 3'- agCCAGUU-----------CUGGUU----------CUGGCUA-UGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 19562 | 0.67 | 0.862794 |
Target: 5'- cUUGGUCuuGACCGAGAacacUCGGUcguccucguACCAGa -3' miRNA: 3'- -AGCCAGuuCUGGUUCU----GGCUA---------UGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 17008 | 0.67 | 0.862794 |
Target: 5'- aCGGcaUCAAGgcggcaGCCGAGACCauGAUGCCc- -3' miRNA: 3'- aGCC--AGUUC------UGGUUCUGG--CUAUGGuc -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 31618 | 0.67 | 0.862794 |
Target: 5'- cCGGUUcGGGCC--GACCGGaGCCAa -3' miRNA: 3'- aGCCAGuUCUGGuuCUGGCUaUGGUc -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 44375 | 0.67 | 0.862794 |
Target: 5'- uUCGaGUcCGGGACUAcaAGACCGGgaaccaACCGGg -3' miRNA: 3'- -AGC-CA-GUUCUGGU--UCUGGCUa-----UGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 21285 | 0.67 | 0.854253 |
Target: 5'- -aGGUCAgcAGAUCGccACCGAUguucACCAGg -3' miRNA: 3'- agCCAGU--UCUGGUucUGGCUA----UGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 41184 | 0.67 | 0.854253 |
Target: 5'- cUCGGgugaUCGAGACCAAGGuCCacgGCCGc -3' miRNA: 3'- -AGCC----AGUUCUGGUUCU-GGcuaUGGUc -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 36447 | 0.67 | 0.854253 |
Target: 5'- cUCGGcCAAGGCCAGgGAUCucuuCCAGg -3' miRNA: 3'- -AGCCaGUUCUGGUU-CUGGcuauGGUC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 40678 | 0.67 | 0.845465 |
Target: 5'- aUGGUgAGGGCUggGGCUGAUgucuGCCuGg -3' miRNA: 3'- aGCCAgUUCUGGuuCUGGCUA----UGGuC- -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 4007 | 0.67 | 0.845465 |
Target: 5'- gUGGUCGAG-CCAggcaaGGACCGccugACCAu -3' miRNA: 3'- aGCCAGUUCuGGU-----UCUGGCua--UGGUc -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 12516 | 0.67 | 0.845465 |
Target: 5'- aUCGGUCAgaccaccaAGGCCAucucGAuugcugacaccaCCGGUGCCAc -3' miRNA: 3'- -AGCCAGU--------UCUGGUu---CU------------GGCUAUGGUc -5' |
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7308 | 3' | -51.3 | NC_001900.1 | + | 1930 | 0.67 | 0.845465 |
Target: 5'- uUCGGauUCGcccgcAGGCCGAGGCCGucacCCGGc -3' miRNA: 3'- -AGCC--AGU-----UCUGGUUCUGGCuau-GGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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