Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7309 | 3' | -52.6 | NC_001900.1 | + | 21417 | 0.66 | 0.841835 |
Target: 5'- gACAUCGuCACCGGccUGGGCgacUUCCa -3' miRNA: 3'- aUGUAGC-GUGGCUucACCUGgc-AAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 2592 | 0.66 | 0.832781 |
Target: 5'- gGCAgaUCGCuugcagguuagACCGcGAGUGG-UCGUUCCc -3' miRNA: 3'- aUGU--AGCG-----------UGGC-UUCACCuGGCAAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 35109 | 0.66 | 0.832781 |
Target: 5'- -uCGUCGCAgaCGAuGUGGACU--UCCa -3' miRNA: 3'- auGUAGCGUg-GCUuCACCUGGcaAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 42253 | 0.66 | 0.823506 |
Target: 5'- aACGUcCGCACCGAgucGGgGGAcggggaugacCCGUUCa -3' miRNA: 3'- aUGUA-GCGUGGCU---UCaCCU----------GGCAAGg -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 34661 | 0.66 | 0.823506 |
Target: 5'- aACAUCucCGUCGAGGUGGACgaugCGUUCUg -3' miRNA: 3'- aUGUAGc-GUGGCUUCACCUG----GCAAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 22317 | 0.66 | 0.823506 |
Target: 5'- -cCAUCGCuggGCCGggGcGG-UCGUUCUu -3' miRNA: 3'- auGUAGCG---UGGCuuCaCCuGGCAAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 9133 | 0.66 | 0.814019 |
Target: 5'- cGCA-CGgGCCGGguGGUGG-CCG-UCCg -3' miRNA: 3'- aUGUaGCgUGGCU--UCACCuGGCaAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 36874 | 0.66 | 0.814019 |
Target: 5'- -cCGUUGCACCcuuGAGGUacuGGGCCaGUUUCa -3' miRNA: 3'- auGUAGCGUGG---CUUCA---CCUGG-CAAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 44241 | 0.66 | 0.804332 |
Target: 5'- gGCAagGCACCGAAGccGACCaagCCc -3' miRNA: 3'- aUGUagCGUGGCUUCacCUGGcaaGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 3029 | 0.67 | 0.763807 |
Target: 5'- -uCAUCGCACCGA--UGGGCUug-CCc -3' miRNA: 3'- auGUAGCGUGGCUucACCUGGcaaGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 39779 | 0.68 | 0.721014 |
Target: 5'- -uCAUCGCGCUGGcucuGcUGGAuCUGUUCCc -3' miRNA: 3'- auGUAGCGUGGCUu---C-ACCU-GGCAAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 38044 | 0.69 | 0.630622 |
Target: 5'- cUACAcCGCAUCGGAGaccGGACUGUgagugaaUCCa -3' miRNA: 3'- -AUGUaGCGUGGCUUCa--CCUGGCA-------AGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 43556 | 0.72 | 0.499202 |
Target: 5'- gGCggCGCACCGAGcUGGccgGCCGaUCCa -3' miRNA: 3'- aUGuaGCGUGGCUUcACC---UGGCaAGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 24032 | 0.72 | 0.488638 |
Target: 5'- gACGaCGCcgguuUCGAGGUGGACCGagCCg -3' miRNA: 3'- aUGUaGCGu----GGCUUCACCUGGCaaGG- -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 13049 | 0.74 | 0.37141 |
Target: 5'- gACAUC-CACCgGGAGgGGGCCGUUCa -3' miRNA: 3'- aUGUAGcGUGG-CUUCaCCUGGCAAGg -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 22113 | 0.75 | 0.353816 |
Target: 5'- aACAaCGCAgCGAGGUGGGCCGa--- -3' miRNA: 3'- aUGUaGCGUgGCUUCACCUGGCaagg -5' |
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7309 | 3' | -52.6 | NC_001900.1 | + | 19634 | 1.1 | 0.001504 |
Target: 5'- gUACAUCGCACCGAAGUGGACCGUUCCc -3' miRNA: 3'- -AUGUAGCGUGGCUUCACCUGGCAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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