Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 731 | 3' | -62.6 | AC_000020.1 | + | 3037 | 0.67 | 0.195511 |
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Target: 5'- gUUCagaCaCAGCACCCCU---UCCCUGCg -3' miRNA: 3'- -AAGg--GcGUCGUGGGGGagcAGGGACG- -5' |
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| 731 | 3' | -62.6 | AC_000020.1 | + | 24643 | 0.68 | 0.148854 |
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Target: 5'- aUCCC-CAGCGCCUCCUgGaucaggCCCgacgGCa -3' miRNA: 3'- aAGGGcGUCGUGGGGGAgCa-----GGGa---CG- -5' |
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| 731 | 3' | -62.6 | AC_000020.1 | + | 11186 | 0.69 | 0.129533 |
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Target: 5'- -aCCCc---CGCCCCCUCGUCCCgccuaaugGCa -3' miRNA: 3'- aaGGGcgucGUGGGGGAGCAGGGa-------CG- -5' |
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| 731 | 3' | -62.6 | AC_000020.1 | + | 14220 | 0.7 | 0.106361 |
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Target: 5'- -gCCCGCGGgGCCCCuCUUGaCCCUa- -3' miRNA: 3'- aaGGGCGUCgUGGGG-GAGCaGGGAcg -5' |
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| 731 | 3' | -62.6 | AC_000020.1 | + | 9939 | 0.72 | 0.073316 |
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Target: 5'- cUCUggaGCAGCACCCCCUUGUaagcgggccaaaCCCUcGCu -3' miRNA: 3'- aAGGg--CGUCGUGGGGGAGCA------------GGGA-CG- -5' |
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| 731 | 3' | -62.6 | AC_000020.1 | + | 10395 | 1.09 | 7.9e-05 |
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Target: 5'- uUUCCCGCAGCACCCCCUCGUCCCUGCa -3' miRNA: 3'- -AAGGGCGUCGUGGGGGAGCAGGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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