miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
731 5' -48.5 AC_000020.1 + 4314 0.68 0.82039
Target:  5'- ---cGGGGAcuauaAGCUGGGCAGacaUGAGGu -3'
miRNA:   3'- gauaUUCUUuc---UCGACCCGUC---ACUCC- -5'
731 5' -48.5 AC_000020.1 + 2465 0.68 0.788935
Target:  5'- aUGUuuGGAccuuAGGGCggGGGCggaGGUGAGGg -3'
miRNA:   3'- gAUAuuCUU----UCUCGa-CCCG---UCACUCC- -5'
731 5' -48.5 AC_000020.1 + 11708 0.71 0.625216
Target:  5'- ---aGAGGucagcAGGGCcaUGGGCAGUGAGa -3'
miRNA:   3'- gauaUUCUu----UCUCG--ACCCGUCACUCc -5'
731 5' -48.5 AC_000020.1 + 8262 0.75 0.429341
Target:  5'- ----uAGAAAGGGCUGGGCua-GGGGa -3'
miRNA:   3'- gauauUCUUUCUCGACCCGucaCUCC- -5'
731 5' -48.5 AC_000020.1 + 7720 0.79 0.252114
Target:  5'- uCUAUGAGGgcGGGCagguugUGGGCAGUGAuGGg -3'
miRNA:   3'- -GAUAUUCUuuCUCG------ACCCGUCACU-CC- -5'
731 5' -48.5 AC_000020.1 + 10361 1.14 0.001154
Target:  5'- aCUAUAAGAAAGAGCUGGGCAGUGAGGg -3'
miRNA:   3'- -GAUAUUCUUUCUCGACCCGUCACUCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.