Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 731 | 5' | -48.5 | AC_000020.1 | + | 4314 | 0.68 | 0.82039 |
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Target: 5'- ---cGGGGAcuauaAGCUGGGCAGacaUGAGGu -3' miRNA: 3'- gauaUUCUUuc---UCGACCCGUC---ACUCC- -5' |
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| 731 | 5' | -48.5 | AC_000020.1 | + | 2465 | 0.68 | 0.788935 |
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Target: 5'- aUGUuuGGAccuuAGGGCggGGGCggaGGUGAGGg -3' miRNA: 3'- gAUAuuCUU----UCUCGa-CCCG---UCACUCC- -5' |
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| 731 | 5' | -48.5 | AC_000020.1 | + | 11708 | 0.71 | 0.625216 |
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Target: 5'- ---aGAGGucagcAGGGCcaUGGGCAGUGAGa -3' miRNA: 3'- gauaUUCUu----UCUCG--ACCCGUCACUCc -5' |
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| 731 | 5' | -48.5 | AC_000020.1 | + | 8262 | 0.75 | 0.429341 |
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Target: 5'- ----uAGAAAGGGCUGGGCua-GGGGa -3' miRNA: 3'- gauauUCUUUCUCGACCCGucaCUCC- -5' |
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| 731 | 5' | -48.5 | AC_000020.1 | + | 7720 | 0.79 | 0.252114 |
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Target: 5'- uCUAUGAGGgcGGGCagguugUGGGCAGUGAuGGg -3' miRNA: 3'- -GAUAUUCUuuCUCG------ACCCGUCACU-CC- -5' |
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| 731 | 5' | -48.5 | AC_000020.1 | + | 10361 | 1.14 | 0.001154 |
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Target: 5'- aCUAUAAGAAAGAGCUGGGCAGUGAGGg -3' miRNA: 3'- -GAUAUUCUUUCUCGACCCGUCACUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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