miRNA: 3'- gggGCCGGGUCGCUA-CC-GGC-----------UCCAGC- -5'
miRNA: 3'- ggGGCCG-GGUCGCUAccGGCUCCAGC- -5'
miRNA: 3'- gGG-GCCGG-GUCGCUac----CGGCU----------CCAGC- -5'
miRNA: 3'- ggGGCCGGGUcGcuaCCGGCUCCagc -5'
miRNA: 3'- -GGG-GcCGGGuCGCUaCCGgCUCCAGC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7313 | 3' | -63.3 | NC_001900.1 | + | 27965 | 0.66 | 0.332328 |
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Target: 5'- --gCGGCCCAGCGAUcGGaCUGugaaaggagcaAGGUUGg -3' miRNA: 3'- gggGCCGGGUCGCUA-CC-GGC-----------UCCAGC- -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 5808 | 0.66 | 0.347849 |
|
Target: 5'- -aCCGGCgUCGGCGGUcaUCGAGGUUGg -3' miRNA: 3'- ggGGCCG-GGUCGCUAccGGCUCCAGC- -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 40381 | 0.66 | 0.347849 |
|
Target: 5'- aCCaCGGCCgCAGCGGcucacuGCCGAccggccacacGGUCGa -3' miRNA: 3'- gGG-GCCGG-GUCGCUac----CGGCU----------CCAGC- -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 310 | 0.66 | 0.347849 |
|
Target: 5'- -aCCGGCCCAaCaagGGCCGGGGa-- -3' miRNA: 3'- ggGGCCGGGUcGcuaCCGGCUCCagc -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 43211 | 0.66 | 0.347849 |
|
Target: 5'- uCCCaCaGCUCcGCGA-GGUgGAGGUCGu -3' miRNA: 3'- -GGG-GcCGGGuCGCUaCCGgCUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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