Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7313 | 3' | -63.3 | NC_001900.1 | + | 26069 | 0.7 | 0.167155 |
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Target: 5'- aUCCCGGCCCAccacGCccgGAcaagGGCgGGGGUCa -3' miRNA: 3'- -GGGGCCGGGU----CG---CUa---CCGgCUCCAGc -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 40361 | 0.7 | 0.171516 |
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Target: 5'- aCCCGuGCUCGGCGA--GCCG-GGUCa -3' miRNA: 3'- gGGGC-CGGGUCGCUacCGGCuCCAGc -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 33170 | 0.7 | 0.182399 |
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Target: 5'- gCCCUGGCCCAGCu-UGGCaaccuCGucgggaucaacgcccAGGUCGa -3' miRNA: 3'- -GGGGCCGGGUCGcuACCG-----GC---------------UCCAGC- -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 43255 | 0.69 | 0.220965 |
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Target: 5'- gUCUCGGUCCuccgcuaccuuGGCG-UGGCCGAuguguaGGUCGg -3' miRNA: 3'- -GGGGCCGGG-----------UCGCuACCGGCU------CCAGC- -5' |
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| 7313 | 3' | -63.3 | NC_001900.1 | + | 22252 | 1.09 | 0.000183 |
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Target: 5'- gCCCCGGCCCAGCGAUGGCCGAGGUCGu -3' miRNA: 3'- -GGGGCCGGGUCGCUACCGGCUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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