Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7314 | 3' | -55.9 | NC_001900.1 | + | 39262 | 0.66 | 0.672546 |
Target: 5'- cGGCUgGgCGCGGgauugcauCGAGAugCUCCaGCGAg -3' miRNA: 3'- -UUGAgC-GUGCU--------GCUCUugGAGG-CGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 31740 | 0.66 | 0.672546 |
Target: 5'- cAGCUCGaCACcGCGAGGcggACCUUCgGUGAa -3' miRNA: 3'- -UUGAGC-GUGcUGCUCU---UGGAGG-CGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 25585 | 0.66 | 0.661538 |
Target: 5'- --aUCGguCGACc-GGACCUCCGCa- -3' miRNA: 3'- uugAGCguGCUGcuCUUGGAGGCGcu -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 26696 | 0.66 | 0.661538 |
Target: 5'- ---gCGgGCGACGGGAAUCgaacCCGCGu -3' miRNA: 3'- uugaGCgUGCUGCUCUUGGa---GGCGCu -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 10438 | 0.66 | 0.63945 |
Target: 5'- gAGCuUCGUgGCGGCGucAGCCUUCGCGGc -3' miRNA: 3'- -UUG-AGCG-UGCUGCucUUGGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 35170 | 0.66 | 0.628391 |
Target: 5'- --aUCGuCGCGACGAGGACCa-CGaCGAc -3' miRNA: 3'- uugAGC-GUGCUGCUCUUGGagGC-GCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 34750 | 0.66 | 0.617335 |
Target: 5'- cGGCUCGCAUGGCGAuGAugCaCUggGCGGa -3' miRNA: 3'- -UUGAGCGUGCUGCU-CUugGaGG--CGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 37335 | 0.67 | 0.606292 |
Target: 5'- uGAUcCGCugGGCGAacuCCUCCGaCGAa -3' miRNA: 3'- -UUGaGCGugCUGCUcuuGGAGGC-GCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 20140 | 0.67 | 0.595272 |
Target: 5'- gAGCU-GCGCGACGGcGACUUCCGUc- -3' miRNA: 3'- -UUGAgCGUGCUGCUcUUGGAGGCGcu -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 22678 | 0.67 | 0.584283 |
Target: 5'- cGACUCGacaACG-CGAucacccuCCUCCGCGAg -3' miRNA: 3'- -UUGAGCg--UGCuGCUcuu----GGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 33202 | 0.69 | 0.477871 |
Target: 5'- cAACgcccaGguCGACcAGAGCCUCCGUGAc -3' miRNA: 3'- -UUGag---CguGCUGcUCUUGGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 11179 | 0.69 | 0.467725 |
Target: 5'- aGGCUgGcCGCGAUcugGAGAAgUUCCGCGAa -3' miRNA: 3'- -UUGAgC-GUGCUG---CUCUUgGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 39051 | 0.69 | 0.457691 |
Target: 5'- uAC-CGCugGAUGA---CCUCCGCGAu -3' miRNA: 3'- uUGaGCGugCUGCUcuuGGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 11857 | 0.7 | 0.432157 |
Target: 5'- -uCUUGCAgGGCGAGGacGCCgacucgaucgccgagUCCGCGAa -3' miRNA: 3'- uuGAGCGUgCUGCUCU--UGG---------------AGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 47842 | 0.7 | 0.409341 |
Target: 5'- gGACUCGCGugccgUGACGcGAACCU-CGCGGu -3' miRNA: 3'- -UUGAGCGU-----GCUGCuCUUGGAgGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 46672 | 0.7 | 0.40934 |
Target: 5'- cGACgCGUACGACGAcGGGCUgaccuUCCGUGAg -3' miRNA: 3'- -UUGaGCGUGCUGCU-CUUGG-----AGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 6007 | 0.7 | 0.390911 |
Target: 5'- --aUCgGCGCGAgcCGGuGACCUCCGCGAg -3' miRNA: 3'- uugAG-CGUGCU--GCUcUUGGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 13219 | 0.7 | 0.390004 |
Target: 5'- ---gCGCugGGCGucucgucGGAGaCCUCCGCGAa -3' miRNA: 3'- uugaGCGugCUGC-------UCUU-GGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 25242 | 0.71 | 0.347272 |
Target: 5'- aGGCUCGCcucguaccCGGCccGAGCCUCUGCGAg -3' miRNA: 3'- -UUGAGCGu-------GCUGcuCUUGGAGGCGCU- -5' |
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7314 | 3' | -55.9 | NC_001900.1 | + | 3768 | 0.72 | 0.314948 |
Target: 5'- cGCUCGUuCGAUGAGGucggcgucgucACCUUCGCGGu -3' miRNA: 3'- uUGAGCGuGCUGCUCU-----------UGGAGGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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