Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7314 | 5' | -51.4 | NC_001900.1 | + | 28291 | 0.66 | 0.88685 |
Target: 5'- --aCGAGGccGUCCGCaAGGACGaggCCa -3' miRNA: 3'- caaGUUUCucCAGGCGcUCCUGUa--GG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 14263 | 0.66 | 0.886849 |
Target: 5'- -gUCAAGGAGGa--GCGAGacGGCGUCa -3' miRNA: 3'- caAGUUUCUCCaggCGCUC--CUGUAGg -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 29952 | 0.66 | 0.875923 |
Target: 5'- -aUCGAGGccaugacgugaccgGGGUCaGCGAGGAagcaccgacgcacCGUCCa -3' miRNA: 3'- caAGUUUC--------------UCCAGgCGCUCCU-------------GUAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 25947 | 0.66 | 0.871079 |
Target: 5'- --cCGGGGAGGUguucgagcacuaCCGCGuGGcCGUCUa -3' miRNA: 3'- caaGUUUCUCCA------------GGCGCuCCuGUAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 47932 | 0.66 | 0.862795 |
Target: 5'- cUUCAccGAGcaCCGCGAGGuucGCGUCa -3' miRNA: 3'- cAAGUuuCUCcaGGCGCUCC---UGUAGg -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 7095 | 0.67 | 0.836439 |
Target: 5'- uUUCGAGccGGUCCuCGAGGAU-UCCg -3' miRNA: 3'- cAAGUUUcuCCAGGcGCUCCUGuAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 41479 | 0.67 | 0.827183 |
Target: 5'- --cCAAGGGGGUUggggGCGcAGGGCGUCa -3' miRNA: 3'- caaGUUUCUCCAGg---CGC-UCCUGUAGg -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 20798 | 0.67 | 0.827182 |
Target: 5'- cGUUCGAugaccugaAG-GGUCUGUG-GGGCAUCg -3' miRNA: 3'- -CAAGUU--------UCuCCAGGCGCuCCUGUAGg -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 8319 | 0.67 | 0.827182 |
Target: 5'- --cCGAcGGGuUCCGgGAGGGCAUCg -3' miRNA: 3'- caaGUUuCUCcAGGCgCUCCUGUAGg -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 16358 | 0.67 | 0.817709 |
Target: 5'- -gUCGuAGAuGUCCGCGAGcGCGUaCCg -3' miRNA: 3'- caAGUuUCUcCAGGCGCUCcUGUA-GG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 31646 | 0.67 | 0.817709 |
Target: 5'- --cCGAGcguGAGGUCCG---GGACAUCCg -3' miRNA: 3'- caaGUUU---CUCCAGGCgcuCCUGUAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 45916 | 0.67 | 0.817708 |
Target: 5'- cUUCGAu--GG-CCGCGAGGAUcucgcgGUCCu -3' miRNA: 3'- cAAGUUucuCCaGGCGCUCCUG------UAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 35224 | 0.68 | 0.798149 |
Target: 5'- aUUCGAAGuGGUCCG--AGGGCuucucgGUCCa -3' miRNA: 3'- cAAGUUUCuCCAGGCgcUCCUG------UAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 10486 | 0.68 | 0.77785 |
Target: 5'- ---gGAAGAGG-CCGCGAuGGGCcUCg -3' miRNA: 3'- caagUUUCUCCaGGCGCU-CCUGuAGg -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 35083 | 0.68 | 0.767454 |
Target: 5'- cGUUCGugaucGAGGacucCCGCGAGGGCugggcagCCg -3' miRNA: 3'- -CAAGUuu---CUCCa---GGCGCUCCUGua-----GG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 40116 | 0.69 | 0.724511 |
Target: 5'- -cUCGGGGAGG-CCGUGAccGACAUCg -3' miRNA: 3'- caAGUUUCUCCaGGCGCUc-CUGUAGg -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 18800 | 0.7 | 0.691231 |
Target: 5'- cGUUCuu-GAugcGGUCCcucugguacGCGAGGAUGUCCu -3' miRNA: 3'- -CAAGuuuCU---CCAGG---------CGCUCCUGUAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 854 | 0.7 | 0.691231 |
Target: 5'- --cCGGAGGGGgccacggUCGCGAucagcucgauGGGCAUCCg -3' miRNA: 3'- caaGUUUCUCCa------GGCGCU----------CCUGUAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 18955 | 0.7 | 0.68 |
Target: 5'- -gUCGAucAGGaCCGCGAGGA-AUCCa -3' miRNA: 3'- caAGUUucUCCaGGCGCUCCUgUAGG- -5' |
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7314 | 5' | -51.4 | NC_001900.1 | + | 27781 | 0.7 | 0.66872 |
Target: 5'- --cCAGAGA-GUCgGCGAGGGCAcguacugcaUCCu -3' miRNA: 3'- caaGUUUCUcCAGgCGCUCCUGU---------AGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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