Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7315 | 3' | -58.5 | NC_001900.1 | + | 17839 | 0.66 | 0.4968 |
Target: 5'- uGGUCA-CCUCgCUCgGCccGUACCUGCc -3' miRNA: 3'- -CUAGUgGGAG-GAGgCGcuCGUGGACG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 23078 | 0.66 | 0.486611 |
Target: 5'- aGGUCGCCCUUgggguugaUGcCGAGgGCCUGCa -3' miRNA: 3'- -CUAGUGGGAGgag-----GC-GCUCgUGGACG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 20400 | 0.66 | 0.486611 |
Target: 5'- cAUCAUCC-CgCUCCauCGAGCGCCUuGCu -3' miRNA: 3'- cUAGUGGGaG-GAGGc-GCUCGUGGA-CG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 25225 | 0.67 | 0.466539 |
Target: 5'- ---gGCCCgagCCUCUGCGAGgCuggCUGCg -3' miRNA: 3'- cuagUGGGa--GGAGGCGCUC-Gug-GACG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 14441 | 0.68 | 0.409064 |
Target: 5'- --aCGCCCcguccgggUUCUUCGCGGGCACCg-- -3' miRNA: 3'- cuaGUGGG--------AGGAGGCGCUCGUGGacg -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 8903 | 0.68 | 0.390909 |
Target: 5'- -uUCGCgUUCgaCUGUGAGCGCCUGg -3' miRNA: 3'- cuAGUGgGAGgaGGCGCUCGUGGACg -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 9976 | 0.68 | 0.382031 |
Target: 5'- --aCGCCCaUCagCUCCGC-AGuCGCCUGCa -3' miRNA: 3'- cuaGUGGG-AG--GAGGCGcUC-GUGGACG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 3340 | 0.69 | 0.315976 |
Target: 5'- --cCACCC-CCUggcCUGCGGGCgACCUGUu -3' miRNA: 3'- cuaGUGGGaGGA---GGCGCUCG-UGGACG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 43789 | 0.7 | 0.272342 |
Target: 5'- cGGUCggGCCgCUCCUCgcUGUGAGCGCgaGCg -3' miRNA: 3'- -CUAG--UGG-GAGGAG--GCGCUCGUGgaCG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 6883 | 0.72 | 0.204541 |
Target: 5'- --aCAUCCUgCCUCCGaCGGGCAggcuccacagguuCCUGCa -3' miRNA: 3'- cuaGUGGGA-GGAGGC-GCUCGU-------------GGACG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 28683 | 0.74 | 0.165639 |
Target: 5'- ---uGCCCUCCUCgGCGAuGUACCaggGCa -3' miRNA: 3'- cuagUGGGAGGAGgCGCU-CGUGGa--CG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 6507 | 0.78 | 0.080642 |
Target: 5'- -cUCAgCCUCagcguucugcuuggCCGCGAGCACCUGCu -3' miRNA: 3'- cuAGUgGGAGga------------GGCGCUCGUGGACG- -5' |
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7315 | 3' | -58.5 | NC_001900.1 | + | 22691 | 1.11 | 0.000284 |
Target: 5'- cGAUCACCCUCCUCCGCGAGCACCUGCu -3' miRNA: 3'- -CUAGUGGGAGGAGGCGCUCGUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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