Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7315 | 5' | -56.1 | NC_001900.1 | + | 24837 | 0.66 | 0.614288 |
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Target: 5'- -cGGuGGUGCU--GGAGGcaaUUCGAGGAa -3' miRNA: 3'- gaCCuCCGCGAcgUCUCC---AAGUUCCU- -5' |
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| 7315 | 5' | -56.1 | NC_001900.1 | + | 34860 | 0.66 | 0.592033 |
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Target: 5'- -cGaAGGCGa-GCAGuacAGGUUCGAGGAc -3' miRNA: 3'- gaCcUCCGCgaCGUC---UCCAAGUUCCU- -5' |
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| 7315 | 5' | -56.1 | NC_001900.1 | + | 986 | 0.66 | 0.580954 |
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Target: 5'- cCUGGAGGCcaGC-GCGGAaaagcgccuGGUcgaccuUCGAGGAg -3' miRNA: 3'- -GACCUCCG--CGaCGUCU---------CCA------AGUUCCU- -5' |
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| 7315 | 5' | -56.1 | NC_001900.1 | + | 3526 | 0.66 | 0.580954 |
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Target: 5'- aUGGccgGGGUGUcggUGCcguuGAGGUUCAGGGu -3' miRNA: 3'- gACC---UCCGCG---ACGu---CUCCAAGUUCCu -5' |
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| 7315 | 5' | -56.1 | NC_001900.1 | + | 17421 | 0.67 | 0.558933 |
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Target: 5'- -cGuuGGCGaacucCUGCAGAGGagCGAGGAg -3' miRNA: 3'- gaCcuCCGC-----GACGUCUCCaaGUUCCU- -5' |
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| 7315 | 5' | -56.1 | NC_001900.1 | + | 28239 | 0.68 | 0.494553 |
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Target: 5'- --cGAGGCGCUGCuGGAGaacCAAGGGc -3' miRNA: 3'- gacCUCCGCGACG-UCUCcaaGUUCCU- -5' |
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| 7315 | 5' | -56.1 | NC_001900.1 | + | 22728 | 1.09 | 0.000635 |
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Target: 5'- uCUGGAGGCGCUGCAGAGGUUCAAGGAg -3' miRNA: 3'- -GACCUCCGCGACGUCUCCAAGUUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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