Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7316 | 5' | -59.1 | NC_001900.1 | + | 8610 | 0.68 | 0.361705 |
Target: 5'- gGUCCaaguCCAacgacuggacGGCUCUGGUCGGCugccgGGUCUc -3' miRNA: 3'- -UAGGg---GGU----------CCGAGACCAGUUG-----CCAGA- -5' |
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7316 | 5' | -59.1 | NC_001900.1 | + | 10302 | 0.68 | 0.361705 |
Target: 5'- uUCCCCCGGacauGCUCcgcaUGGa-GACGGUCUg -3' miRNA: 3'- uAGGGGGUC----CGAG----ACCagUUGCCAGA- -5' |
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7316 | 5' | -59.1 | NC_001900.1 | + | 12822 | 0.68 | 0.327916 |
Target: 5'- uUCUCCCAGcucaUCUGGggccagaUCGGCGGUCUg -3' miRNA: 3'- uAGGGGGUCcg--AGACC-------AGUUGCCAGA- -5' |
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7316 | 5' | -59.1 | NC_001900.1 | + | 3152 | 0.7 | 0.269553 |
Target: 5'- gGUCCCCCuGGCgg-GGUUcugAGCGGUUg -3' miRNA: 3'- -UAGGGGGuCCGagaCCAG---UUGCCAGa -5' |
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7316 | 5' | -59.1 | NC_001900.1 | + | 22914 | 1.06 | 0.000522 |
Target: 5'- gAUCCCCCAGGCUCUGGUCAACGGUCUg -3' miRNA: 3'- -UAGGGGGUCCGAGACCAGUUGCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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