Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7317 | 3' | -58.7 | NC_001900.1 | + | 28165 | 0.66 | 0.518372 |
Target: 5'- aGCCCUUGGUucuccagCAGCgCCuc-GGCGAg -3' miRNA: 3'- -CGGGAGCCGua-----GUUGgGGuucCCGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 6728 | 0.66 | 0.508035 |
Target: 5'- uGCCguaUCGGCAggcaggcCAGgCCCGAGGG-GAu -3' miRNA: 3'- -CGGg--AGCCGUa------GUUgGGGUUCCCgCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 35721 | 0.66 | 0.494729 |
Target: 5'- uGCCCUUcuucaCGUCGGCCuccgcuggccguagCCAGGGGUGAa -3' miRNA: 3'- -CGGGAGcc---GUAGUUGG--------------GGUUCCCGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 14078 | 0.66 | 0.48763 |
Target: 5'- aUCCUCGGCGcUCAGaCCCAgaucgaguggaAGGGCc- -3' miRNA: 3'- cGGGAGCCGU-AGUUgGGGU-----------UCCCGcu -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 26904 | 0.66 | 0.485611 |
Target: 5'- cGCCgUCGGCGgucuggccuUCAGCCUCAGuuucaccgcgcuGGCGAg -3' miRNA: 3'- -CGGgAGCCGU---------AGUUGGGGUUc-----------CCGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 23132 | 0.66 | 0.477573 |
Target: 5'- gGCCCUgaccgGGCG-CAGCCC---GGGCGAg -3' miRNA: 3'- -CGGGAg----CCGUaGUUGGGguuCCCGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 32408 | 0.66 | 0.46762 |
Target: 5'- gGCCUugUCGGCGUCGgagcccgacaGCUCCGuaaacgccgGGGGCa- -3' miRNA: 3'- -CGGG--AGCCGUAGU----------UGGGGU---------UCCCGcu -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 12303 | 0.67 | 0.448041 |
Target: 5'- gGCCCgUGGUacccAUCGGCaCCUucuGGGCGAa -3' miRNA: 3'- -CGGGaGCCG----UAGUUG-GGGuu-CCCGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 2582 | 0.67 | 0.448041 |
Target: 5'- aGCCCgaCGGCGggaacUCAagGCCCgGAGGaaGCGAc -3' miRNA: 3'- -CGGGa-GCCGU-----AGU--UGGGgUUCC--CGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 37045 | 0.67 | 0.438423 |
Target: 5'- aCCCaccggcaaGGCAUCaAGCCCCAcaccuGGGGCu- -3' miRNA: 3'- cGGGag------CCGUAG-UUGGGGU-----UCCCGcu -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 34080 | 0.67 | 0.438423 |
Target: 5'- cUCCUCGGCcgcucuGUCGACCUCAGcGGCc- -3' miRNA: 3'- cGGGAGCCG------UAGUUGGGGUUcCCGcu -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 11935 | 0.67 | 0.438423 |
Target: 5'- aGCCgguuucCGGUuUCGACCCCAcccAGGGCu- -3' miRNA: 3'- -CGGga----GCCGuAGUUGGGGU---UCCCGcu -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 17235 | 0.67 | 0.428925 |
Target: 5'- aGCCCUUGGCGauguUCAcuAUgCCGGuGGCGAc -3' miRNA: 3'- -CGGGAGCCGU----AGU--UGgGGUUcCCGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 38093 | 0.67 | 0.428925 |
Target: 5'- aGCCgaucaUCGGCuAUCAACCCgaCAAGGuCGAu -3' miRNA: 3'- -CGGg----AGCCG-UAGUUGGG--GUUCCcGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 23310 | 0.67 | 0.428925 |
Target: 5'- -aCCaCGGCgGUCGGCCCCGgcuGGGcCGAg -3' miRNA: 3'- cgGGaGCCG-UAGUUGGGGUu--CCC-GCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 36184 | 0.67 | 0.428925 |
Target: 5'- -gCCUCGGCAgccgAACCCCucuccGGGaCGAc -3' miRNA: 3'- cgGGAGCCGUag--UUGGGGuu---CCC-GCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 15141 | 0.67 | 0.428925 |
Target: 5'- aGCCga-GGCAcgcuccccgaguUCGGCUUCGAGGGCGGc -3' miRNA: 3'- -CGGgagCCGU------------AGUUGGGGUUCCCGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 5446 | 0.67 | 0.419549 |
Target: 5'- -aCCUCGGCAaCAcCCCgGAGGuCGAc -3' miRNA: 3'- cgGGAGCCGUaGUuGGGgUUCCcGCU- -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 42238 | 0.68 | 0.374596 |
Target: 5'- aGUCCUCGuagagcGCGUCGuacGCUgCGAGGGCGu -3' miRNA: 3'- -CGGGAGC------CGUAGU---UGGgGUUCCCGCu -5' |
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7317 | 3' | -58.7 | NC_001900.1 | + | 9942 | 0.68 | 0.366005 |
Target: 5'- aUCUUCGGCAUCAAgCCCGAagagaucGGCGu -3' miRNA: 3'- cGGGAGCCGUAGUUgGGGUUc------CCGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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