Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7318 | 5' | -54.2 | NC_001900.1 | + | 4761 | 0.71 | 0.462883 |
Target: 5'- cGCUGGCaacaGGUACGGCUaCGG-CGGGg- -3' miRNA: 3'- -CGACCGg---CCAUGCUGA-GUCaGCUUga -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 4871 | 0.66 | 0.765248 |
Target: 5'- aCUGGaucggaaaCCGGUACGGCUCGacugaGAGCUu -3' miRNA: 3'- cGACC--------GGCCAUGCUGAGUcag--CUUGA- -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 6389 | 0.71 | 0.433141 |
Target: 5'- -gUGGCCGGUGCGGCUgcgccgacCAGguugaGAGCg -3' miRNA: 3'- cgACCGGCCAUGCUGA--------GUCag---CUUGa -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 15421 | 0.68 | 0.645148 |
Target: 5'- --aGGCCGGUguccgucGCGACgacgCGaUCGAGCUg -3' miRNA: 3'- cgaCCGGCCA-------UGCUGa---GUcAGCUUGA- -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 20316 | 0.69 | 0.568707 |
Target: 5'- cUUGGCCaGGUAgUGGCUCAGcgaGAGCUg -3' miRNA: 3'- cGACCGG-CCAU-GCUGAGUCag-CUUGA- -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 20969 | 0.67 | 0.690497 |
Target: 5'- cCUGGCUGGagACGGCcaGGUCGAAg- -3' miRNA: 3'- cGACCGGCCa-UGCUGagUCAGCUUga -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 23197 | 1.09 | 0.001119 |
Target: 5'- aGCUGGCCGGUACGACUCAGUCGAACUc -3' miRNA: 3'- -CGACCGGCCAUGCUGAGUCAGCUUGA- -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 23279 | 0.68 | 0.646259 |
Target: 5'- cGCUGGCgGGgAUGACU---UCGAGCg -3' miRNA: 3'- -CGACCGgCCaUGCUGAgucAGCUUGa -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 23328 | 0.66 | 0.753851 |
Target: 5'- gGCUGGgccgaguucuauaCCGGcgGCGGCUaCGGaUCGGGCa -3' miRNA: 3'- -CGACC-------------GGCCa-UGCUGA-GUC-AGCUUGa -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 23738 | 0.69 | 0.601805 |
Target: 5'- cGCUGGCaCGGUcgACGGCaUCgagaaGGUCGAGu- -3' miRNA: 3'- -CGACCG-GCCA--UGCUG-AG-----UCAGCUUga -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 23940 | 0.67 | 0.679496 |
Target: 5'- aGC-GGUcuCGGUGCGGCUCGGUCc---- -3' miRNA: 3'- -CGaCCG--GCCAUGCUGAGUCAGcuuga -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 25529 | 0.69 | 0.590734 |
Target: 5'- gGCUcaGGCCGGUGCGACgacCAag-GAGCUg -3' miRNA: 3'- -CGA--CCGGCCAUGCUGa--GUcagCUUGA- -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 33339 | 0.7 | 0.504131 |
Target: 5'- uGCUGGgCaGGgggaucccaggGCGACUCAGUCGGcgucuGCUg -3' miRNA: 3'- -CGACCgG-CCa----------UGCUGAGUCAGCU-----UGA- -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 35136 | 0.68 | 0.657366 |
Target: 5'- cCUGGCCGagGCGGgUCAguuGUCGAGCc -3' miRNA: 3'- cGACCGGCcaUGCUgAGU---CAGCUUGa -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 37340 | 0.66 | 0.754894 |
Target: 5'- uGCgGGCCGuGUACGACggccCuGUCGGc-- -3' miRNA: 3'- -CGaCCGGC-CAUGCUGa---GuCAGCUuga -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 38205 | 0.67 | 0.679496 |
Target: 5'- cGCUggGGUCGaGUACGACgUGGUCGAcCUg -3' miRNA: 3'- -CGA--CCGGC-CAUGCUGaGUCAGCUuGA- -5' |
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7318 | 5' | -54.2 | NC_001900.1 | + | 45434 | 0.66 | 0.774448 |
Target: 5'- --cGGCCaugccggGGUAgGGCUCaauguuggGGUCGAACUc -3' miRNA: 3'- cgaCCGG-------CCAUgCUGAG--------UCAGCUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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