Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7319 | 3' | -51.8 | NC_001900.1 | + | 4216 | 0.66 | 0.894413 |
Target: 5'- gGGUCACCGccggccGCUucGCCGGucggcAGAUGAa- -3' miRNA: 3'- gCCAGUGGC------UGA--UGGUCu----UCUACUgc -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 29586 | 0.66 | 0.887015 |
Target: 5'- gCGGUcCACCGGCaGCCgccggcccaucgGGuuGAUGAUGu -3' miRNA: 3'- -GCCA-GUGGCUGaUGG------------UCuuCUACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 15939 | 0.66 | 0.88626 |
Target: 5'- aGGUCuuCCGGCUGaucgccacgaagcCCAGAAGcUGAUc -3' miRNA: 3'- gCCAGu-GGCUGAU-------------GGUCUUCuACUGc -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 30022 | 0.66 | 0.884743 |
Target: 5'- cCGGUCACgucauggccucgauCGACUACCAGGcgcaGGAgcuucgcguccUGGCc -3' miRNA: 3'- -GCCAGUG--------------GCUGAUGGUCU----UCU-----------ACUGc -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 7137 | 0.66 | 0.879345 |
Target: 5'- aGGUCGCCaucgGCCGGAucGUGAUGa -3' miRNA: 3'- gCCAGUGGcugaUGGUCUucUACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 14044 | 0.66 | 0.871409 |
Target: 5'- -cGUCguGCCGACgUACCGGAcuGGuUGGCGa -3' miRNA: 3'- gcCAG--UGGCUG-AUGGUCU--UCuACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 7449 | 0.67 | 0.846077 |
Target: 5'- aCGGUCgACgGAgauuggcauCUGCCGGAucccaAGAUGACc -3' miRNA: 3'- -GCCAG-UGgCU---------GAUGGUCU-----UCUACUGc -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 15189 | 0.67 | 0.818635 |
Target: 5'- gGuGUgGCCGACgcucgACCAGccGGUGGCu -3' miRNA: 3'- gC-CAgUGGCUGa----UGGUCuuCUACUGc -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 8024 | 0.68 | 0.7993 |
Target: 5'- gCGGcCACCGGCcg-CGGAguAGAUGAUGa -3' miRNA: 3'- -GCCaGUGGCUGaugGUCU--UCUACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 3174 | 0.68 | 0.789353 |
Target: 5'- gCGGUUgccgcuACCGACUgcucccccGCCGGggGuUGACc -3' miRNA: 3'- -GCCAG------UGGCUGA--------UGGUCuuCuACUGc -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 41152 | 0.68 | 0.779235 |
Target: 5'- aGGUCACCGGCcugGCCcgcaacgucucGGAGGAgaACGu -3' miRNA: 3'- gCCAGUGGCUGa--UGG-----------UCUUCUacUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 6076 | 0.68 | 0.778214 |
Target: 5'- aGGUCACCGGCUcgcGCCGauuccucGAcguAGcgGGCGu -3' miRNA: 3'- gCCAGUGGCUGA---UGGU-------CU---UCuaCUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 9038 | 0.69 | 0.73729 |
Target: 5'- aGGUCACCaagGACU-CCagcaAGAAGAUcGACGc -3' miRNA: 3'- gCCAGUGG---CUGAuGG----UCUUCUA-CUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 19420 | 0.69 | 0.73729 |
Target: 5'- aGG-UACCGGgUACCGGAGuccgggguGGUGACGu -3' miRNA: 3'- gCCaGUGGCUgAUGGUCUU--------CUACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 3864 | 0.71 | 0.626369 |
Target: 5'- aGGUCacGCCGACcGC--GAAGGUGACGa -3' miRNA: 3'- gCCAG--UGGCUGaUGguCUUCUACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 37471 | 0.72 | 0.54721 |
Target: 5'- gCGGUCAUCGAacgggauCUGCguGucgGAGAUGACGa -3' miRNA: 3'- -GCCAGUGGCU-------GAUGguC---UUCUACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 25689 | 0.73 | 0.494423 |
Target: 5'- aCGGUCACCGGCcGCUu--GGAccUGACGg -3' miRNA: 3'- -GCCAGUGGCUGaUGGucuUCU--ACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 32798 | 0.73 | 0.483929 |
Target: 5'- aGG-CACCGACUACguCGGu-GAUGACGa -3' miRNA: 3'- gCCaGUGGCUGAUG--GUCuuCUACUGC- -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 3412 | 0.74 | 0.443082 |
Target: 5'- aGGUCGCCcGCagGCCAGggGGUGGg- -3' miRNA: 3'- gCCAGUGGcUGa-UGGUCuuCUACUgc -5' |
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7319 | 3' | -51.8 | NC_001900.1 | + | 3723 | 0.74 | 0.441091 |
Target: 5'- aCGGUCACCGACacccugaacuuCCAGcucAAGGUGACc -3' miRNA: 3'- -GCCAGUGGCUGau---------GGUC---UUCUACUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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