Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 732 | 3' | -51.1 | AC_000020.1 | + | 12114 | 0.67 | 0.701187 |
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Target: 5'- aUAGCUcuuuGGaGCUGACGC---GCCAGg -3' miRNA: 3'- -GUCGAauu-CC-CGACUGUGuuuCGGUC- -5' |
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| 732 | 3' | -51.1 | AC_000020.1 | + | 19900 | 0.68 | 0.628226 |
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Target: 5'- aCAGCUuugaguaUGAGGGCcuacucaGGCGCAgcGCCAu -3' miRNA: 3'- -GUCGA-------AUUCCCGa------CUGUGUuuCGGUc -5' |
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| 732 | 3' | -51.1 | AC_000020.1 | + | 25399 | 0.69 | 0.564821 |
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Target: 5'- gGGCUUGGGGGCcaaugucaguuggaUaaacucuGACACA-GGCCAGg -3' miRNA: 3'- gUCGAAUUCCCG--------------A-------CUGUGUuUCGGUC- -5' |
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| 732 | 3' | -51.1 | AC_000020.1 | + | 17170 | 0.69 | 0.554192 |
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Target: 5'- cCAGCUUGcggugggagacaacAGGGUgcugGACAU--GGCCAGu -3' miRNA: 3'- -GUCGAAU--------------UCCCGa---CUGUGuuUCGGUC- -5' |
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| 732 | 3' | -51.1 | AC_000020.1 | + | 16019 | 0.77 | 0.181199 |
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Target: 5'- gCAGCUaucaaagggaUGAGGGCUGGCAaAAAGCCu- -3' miRNA: 3'- -GUCGA----------AUUCCCGACUGUgUUUCGGuc -5' |
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| 732 | 3' | -51.1 | AC_000020.1 | + | 11412 | 1.09 | 0.001102 |
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Target: 5'- gCAGCUUAAGGGCUGACACAAAGCCAGu -3' miRNA: 3'- -GUCGAAUUCCCGACUGUGUUUCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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