Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
732 | 5' | -61 | AC_000020.1 | + | 21489 | 0.66 | 0.276353 |
Target: 5'- cCCCCGCgCCuugagGGUGGcGguGGCu -3' miRNA: 3'- cGGGGCGaGGuguaaCCGCC-CguCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 17983 | 0.66 | 0.274948 |
Target: 5'- aGCCUgGUUCCACAUggaaaaguaGCGGGaccugucugugaGGGCa -3' miRNA: 3'- -CGGGgCGAGGUGUAac-------CGCCCg-----------UCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 12564 | 0.67 | 0.242923 |
Target: 5'- cGCCCCaGCUUgggGCGUaGGUGGGCaaagGGGUu -3' miRNA: 3'- -CGGGG-CGAGg--UGUAaCCGCCCG----UCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 15039 | 0.67 | 0.236655 |
Target: 5'- uGCCUCGCcaggacgcgCCACGggcuccUGGgGGGUgauGGGCa -3' miRNA: 3'- -CGGGGCGa--------GGUGUa-----ACCgCCCG---UCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 2646 | 0.68 | 0.218661 |
Target: 5'- -aCCUGCUCUAUuaugcUGGgGGGUcaGGGCa -3' miRNA: 3'- cgGGGCGAGGUGua---ACCgCCCG--UCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 14728 | 0.68 | 0.196499 |
Target: 5'- cGCUuuGCUCCccgGCGUcccuaccagUGGCGGGgucGGCg -3' miRNA: 3'- -CGGggCGAGG---UGUA---------ACCGCCCgu-CCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 9560 | 0.7 | 0.158012 |
Target: 5'- cCCCCGCcCCACcuuUUGGUGGGCu--- -3' miRNA: 3'- cGGGGCGaGGUGu--AACCGCCCGuccg -5' |
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732 | 5' | -61 | AC_000020.1 | + | 9898 | 0.7 | 0.153711 |
Target: 5'- uCUCUGCUUCuagcuugcugGCAgaGGCGGGCAGGg -3' miRNA: 3'- cGGGGCGAGG----------UGUaaCCGCCCGUCCg -5' |
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732 | 5' | -61 | AC_000020.1 | + | 27300 | 0.7 | 0.141436 |
Target: 5'- aGCUCCGCUCUGCAc-GGCaaGGCcGGCa -3' miRNA: 3'- -CGGGGCGAGGUGUaaCCGc-CCGuCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 6801 | 0.7 | 0.139869 |
Target: 5'- gGCCCUGCUCC-CAgugcucguaacucUUGGCaGGCacguagcugggguuAGGCa -3' miRNA: 3'- -CGGGGCGAGGuGU-------------AACCGcCCG--------------UCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 4952 | 0.74 | 0.067387 |
Target: 5'- uGCCCuUGC-CCACGUgaUGGCacuuaGGGCAGGUg -3' miRNA: 3'- -CGGG-GCGaGGUGUA--ACCG-----CCCGUCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 6015 | 0.76 | 0.051829 |
Target: 5'- uGCCCCcauuuuGCUCaCGCAa-GGgGGGCAGGCu -3' miRNA: 3'- -CGGGG------CGAG-GUGUaaCCgCCCGUCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 7713 | 0.77 | 0.046091 |
Target: 5'- gGCCUC-CUCUAUGagGGCGGGCAGGUu -3' miRNA: 3'- -CGGGGcGAGGUGUaaCCGCCCGUCCG- -5' |
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732 | 5' | -61 | AC_000020.1 | + | 11373 | 1.01 | 0.000518 |
Target: 5'- gGCCCCGCU-CACAUUGGCGGGCAGGCg -3' miRNA: 3'- -CGGGGCGAgGUGUAACCGCCCGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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