Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7320 | 3' | -57.8 | NC_001900.1 | + | 3133 | 0.66 | 0.543898 |
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Target: 5'- gCCGGUCGG--GUCGGCG--GGUgcCCg -3' miRNA: 3'- -GGCCAGCCgaUAGCCGCgaCCGaaGG- -5' |
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| 7320 | 3' | -57.8 | NC_001900.1 | + | 43927 | 0.67 | 0.512713 |
|
Target: 5'- aCCaGUCGGCccagcccUC-GCGCUGGCcgUUCCc -3' miRNA: 3'- -GGcCAGCCGau-----AGcCGCGACCG--AAGG- -5' |
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| 7320 | 3' | -57.8 | NC_001900.1 | + | 27451 | 0.67 | 0.482289 |
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Target: 5'- cCCGGUagcacccucugGGCUcuggaGGgGCUGGCUUCg -3' miRNA: 3'- -GGCCAg----------CCGAuag--CCgCGACCGAAGg -5' |
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| 7320 | 3' | -57.8 | NC_001900.1 | + | 39799 | 0.68 | 0.472342 |
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Target: 5'- cCCGGagcuggCGGCUGaucUCaucGCGCUGGCU-CUg -3' miRNA: 3'- -GGCCa-----GCCGAU---AGc--CGCGACCGAaGG- -5' |
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| 7320 | 3' | -57.8 | NC_001900.1 | + | 23469 | 0.68 | 0.462499 |
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Target: 5'- cCUGGUCGGUccacUCGGCcugaUGGCcgUCCu -3' miRNA: 3'- -GGCCAGCCGau--AGCCGcg--ACCGa-AGG- -5' |
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| 7320 | 3' | -57.8 | NC_001900.1 | + | 44779 | 0.66 | 0.597097 |
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Target: 5'- gUGGcagCGGCcagCGGC-CUGGCUgCCg -3' miRNA: 3'- gGCCa--GCCGauaGCCGcGACCGAaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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