Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7320 | 5' | -58.8 | NC_001900.1 | + | 17701 | 0.66 | 0.488472 |
Target: 5'- cGGGcgcucugcAGGCUcugGGGCCUGUgcuGACCGugGu -3' miRNA: 3'- aCCCu-------UCCGG---UCCGGGCA---UUGGCugU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 1064 | 0.66 | 0.478372 |
Target: 5'- cGGGAAGGUucaugucgucgCAGuaCCGcAGCCGGCc -3' miRNA: 3'- aCCCUUCCG-----------GUCcgGGCaUUGGCUGu -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 25002 | 0.67 | 0.435222 |
Target: 5'- gUGGGuaucaccgguuuGGCCGGuggucccgguGCCCGUGGCCaGGCGu -3' miRNA: 3'- -ACCCuu----------CCGGUC----------CGGGCAUUGG-CUGU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 4050 | 0.67 | 0.429513 |
Target: 5'- cGGuGAuGGCCuucgaGGuGCCCGUgaGACCGGCc -3' miRNA: 3'- aCC-CUuCCGG-----UC-CGGGCA--UUGGCUGu -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 17248 | 0.67 | 0.429513 |
Target: 5'- cUGGcGAguucuucaccGGGCuCGGGCCgGUcaucucGACCGGCAc -3' miRNA: 3'- -ACC-CU----------UCCG-GUCCGGgCA------UUGGCUGU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 6791 | 0.67 | 0.420097 |
Target: 5'- cGGGAAGGCCgcagcgGGGUa-GUuGCCGAUg -3' miRNA: 3'- aCCCUUCCGG------UCCGggCAuUGGCUGu -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 41085 | 0.68 | 0.383716 |
Target: 5'- ----cGGGCCAGGCCgGUGACCucGCGg -3' miRNA: 3'- acccuUCCGGUCCGGgCAUUGGc-UGU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 15693 | 0.68 | 0.361217 |
Target: 5'- gGGGGAGGCaAGGCCCGccaaggaAACCucccccuccgggguaGACAg -3' miRNA: 3'- aCCCUUCCGgUCCGGGCa------UUGG---------------CUGU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 15207 | 0.68 | 0.357839 |
Target: 5'- cGGGAGcGuGCCucGGCUggUGUGGCCGACGc -3' miRNA: 3'- aCCCUU-C-CGGu-CCGG--GCAUUGGCUGU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 43845 | 0.69 | 0.309845 |
Target: 5'- cGGGAAcGGCCAGcGCgaGggcugGGCCGACu -3' miRNA: 3'- aCCCUU-CCGGUC-CGggCa----UUGGCUGu -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 44251 | 0.71 | 0.247314 |
Target: 5'- gGGGAagaaGGGCaAGGCaCCGaAGCCGACc -3' miRNA: 3'- aCCCU----UCCGgUCCG-GGCaUUGGCUGu -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 5628 | 0.71 | 0.234921 |
Target: 5'- cGGGcguGGGGaCCAGGCCCcu-GCCGugAg -3' miRNA: 3'- aCCC---UUCC-GGUCCGGGcauUGGCugU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 3084 | 0.71 | 0.228922 |
Target: 5'- cGGGAAGGUCGuugaCGUGACCGACGu -3' miRNA: 3'- aCCCUUCCGGUccggGCAUUGGCUGU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 214 | 0.72 | 0.195602 |
Target: 5'- ----cGGGCCuguauGGCCCGUAGCCGAUu -3' miRNA: 3'- acccuUCCGGu----CCGGGCAUUGGCUGu -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 6699 | 0.74 | 0.141513 |
Target: 5'- gGGGAucGGGCU-GGCCCGU-GCCGugAa -3' miRNA: 3'- aCCCU--UCCGGuCCGGGCAuUGGCugU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 27174 | 0.76 | 0.104325 |
Target: 5'- cUGGGcguGGGCCAcguuGCCCGcGACCGACAu -3' miRNA: 3'- -ACCCu--UCCGGUc---CGGGCaUUGGCUGU- -5' |
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7320 | 5' | -58.8 | NC_001900.1 | + | 23855 | 1 | 0.001852 |
Target: 5'- cUGGGAAGGCCAGGCCCGUAACCG-CAa -3' miRNA: 3'- -ACCCUUCCGGUCCGGGCAUUGGCuGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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