Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7322 | 3' | -55.5 | NC_001900.1 | + | 21191 | 0.66 | 0.67761 |
Target: 5'- -cGAGGGgaucUCGGCCcuggugaacaUCGGUGGCGAUCu -3' miRNA: 3'- gaCUUCC----AGUCGG----------AGCCGUUGCUGGu -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 38093 | 0.66 | 0.673189 |
Target: 5'- cCUGGAcguacGUCuuGGCCUCGGCGuugcgcgucagguCGACCAc -3' miRNA: 3'- -GACUUc----CAG--UCGGAGCCGUu------------GCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 38137 | 0.66 | 0.655452 |
Target: 5'- aCUGAAGG-CGuCCUCGGUuccggucaucguGAcCGACCAu -3' miRNA: 3'- -GACUUCCaGUcGGAGCCG------------UU-GCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 4005 | 0.66 | 0.655452 |
Target: 5'- -cGguGGUCgAGCCa-GGCAAgGACCGc -3' miRNA: 3'- gaCuuCCAG-UCGGagCCGUUgCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 33376 | 0.67 | 0.622077 |
Target: 5'- gUGGAGGUgCGGCCU--GCGcCGGCCGa -3' miRNA: 3'- gACUUCCA-GUCGGAgcCGUuGCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 6837 | 0.67 | 0.622077 |
Target: 5'- uCUGcAGGaUCAG-CUCGGCGAcacccuucuCGACCGa -3' miRNA: 3'- -GACuUCC-AGUCgGAGCCGUU---------GCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 16977 | 0.67 | 0.599854 |
Target: 5'- gCUGGAgccgguguGGUC-GCaCUCGGCAugGACg- -3' miRNA: 3'- -GACUU--------CCAGuCG-GAGCCGUugCUGgu -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 13003 | 0.67 | 0.599854 |
Target: 5'- uCUGAccaacguGGUCAGCggCGGCGGCGAg-- -3' miRNA: 3'- -GACUu------CCAGUCGgaGCCGUUGCUggu -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 8043 | 0.67 | 0.577744 |
Target: 5'- -aGGAGGUUgcuGCCUCGau-GCGGCCAc -3' miRNA: 3'- gaCUUCCAGu--CGGAGCcguUGCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 16787 | 0.67 | 0.577744 |
Target: 5'- -aGAAGGgacugagCAGUCUUGGUGAUGACg- -3' miRNA: 3'- gaCUUCCa------GUCGGAGCCGUUGCUGgu -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 25535 | 0.67 | 0.566754 |
Target: 5'- gCUGgcGGcUCAgGCCgguGCGACGACCAa -3' miRNA: 3'- -GACuuCC-AGU-CGGagcCGUUGCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 21474 | 0.67 | 0.566754 |
Target: 5'- aUGAAGGcguUCAcccUCUCGGCGuucGCGGCCAu -3' miRNA: 3'- gACUUCC---AGUc--GGAGCCGU---UGCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 6315 | 0.67 | 0.566754 |
Target: 5'- cCUGGucGGcgCAGCCgcacCGGCcACGGCCGc -3' miRNA: 3'- -GACUu-CCa-GUCGGa---GCCGuUGCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 16721 | 0.68 | 0.512768 |
Target: 5'- uUGAA-GUCGGCCUUGGCcugGGCCGc -3' miRNA: 3'- gACUUcCAGUCGGAGCCGuugCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 24637 | 0.69 | 0.502215 |
Target: 5'- --cAAGG-CGGCUUCGGCGguuccugggugACGGCCAc -3' miRNA: 3'- gacUUCCaGUCGGAGCCGU-----------UGCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 1601 | 0.69 | 0.502215 |
Target: 5'- gCUGuucGAGGUCGGCuuCUCGGCcgguGGCGACa- -3' miRNA: 3'- -GAC---UUCCAGUCG--GAGCCG----UUGCUGgu -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 28674 | 0.69 | 0.491757 |
Target: 5'- -gGAAGGUgAuGCCcuccUCGGCGAUGuACCAg -3' miRNA: 3'- gaCUUCCAgU-CGG----AGCCGUUGC-UGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 23314 | 0.69 | 0.491757 |
Target: 5'- -cGgcGGUCGGCCcCGGCug-GGCCGa -3' miRNA: 3'- gaCuuCCAGUCGGaGCCGuugCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 9597 | 0.69 | 0.469116 |
Target: 5'- uCUGggGGUCAGgg-CGGCugaucgugauguACGACCGg -3' miRNA: 3'- -GACuuCCAGUCggaGCCGu-----------UGCUGGU- -5' |
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7322 | 3' | -55.5 | NC_001900.1 | + | 45018 | 0.69 | 0.461015 |
Target: 5'- ----uGGUCGGCCuucagaaucuccUCGGCGACaGGCCGa -3' miRNA: 3'- gacuuCCAGUCGG------------AGCCGUUG-CUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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