Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7322 | 3' | -55.5 | NC_001900.1 | + | 26976 | 0.7 | 0.421672 |
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Target: 5'- gCUGAAGGcCAGaCCgccgaCGGCGACGGuuCCGg -3' miRNA: 3'- -GACUUCCaGUC-GGa----GCCGUUGCU--GGU- -5' |
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| 7322 | 3' | -55.5 | NC_001900.1 | + | 23135 | 0.7 | 0.421672 |
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Target: 5'- cCUGAccGGGcgCAGCC-CgGGCGAgGACCAg -3' miRNA: 3'- -GACU--UCCa-GUCGGaG-CCGUUgCUGGU- -5' |
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| 7322 | 3' | -55.5 | NC_001900.1 | + | 38681 | 0.7 | 0.412156 |
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Target: 5'- ---cAGG-CAGCCUCGGUAcaucucAUGACCAg -3' miRNA: 3'- gacuUCCaGUCGGAGCCGU------UGCUGGU- -5' |
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| 7322 | 3' | -55.5 | NC_001900.1 | + | 29071 | 0.7 | 0.402774 |
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Target: 5'- -cGAAGGUCGGCCUccCGGCuucuACuucgaguuccgGGCCAa -3' miRNA: 3'- gaCUUCCAGUCGGA--GCCGu---UG-----------CUGGU- -5' |
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| 7322 | 3' | -55.5 | NC_001900.1 | + | 18422 | 0.72 | 0.299021 |
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Target: 5'- gCUGAGaucGGUCAguucacggccgaggGCUUCGGCAACGGCUu -3' miRNA: 3'- -GACUU---CCAGU--------------CGGAGCCGUUGCUGGu -5' |
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| 7322 | 3' | -55.5 | NC_001900.1 | + | 29236 | 0.74 | 0.258439 |
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Target: 5'- -cGAAGGUCAuCCgaCGGCGACGAgCCAc -3' miRNA: 3'- gaCUUCCAGUcGGa-GCCGUUGCU-GGU- -5' |
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| 7322 | 3' | -55.5 | NC_001900.1 | + | 24459 | 1.09 | 0.000794 |
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Target: 5'- cCUGAAGGUCAGCCUCGGCAACGACCAg -3' miRNA: 3'- -GACUUCCAGUCGGAGCCGUUGCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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