Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7322 | 5' | -57.8 | NC_001900.1 | + | 46669 | 0.66 | 0.598989 |
Target: 5'- cGCGUaCGACgaCGG-GCUGACcuUCCGu -3' miRNA: 3'- uCGCAaGCUGg-GCCaCGACUG--AGGCc -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 41220 | 0.66 | 0.598989 |
Target: 5'- aGGCGaUCGACCCGGaGa--GC-CCGGa -3' miRNA: 3'- -UCGCaAGCUGGGCCaCgacUGaGGCC- -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 23814 | 0.66 | 0.588262 |
Target: 5'- gGGCuccggUCGGCUauCGGcGCUGGCUuCCGGc -3' miRNA: 3'- -UCGca---AGCUGG--GCCaCGACUGA-GGCC- -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 44140 | 0.66 | 0.57757 |
Target: 5'- -aUGUUCGACCCGuUGCcGGaaCCGGa -3' miRNA: 3'- ucGCAAGCUGGGCcACGaCUgaGGCC- -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 22779 | 0.68 | 0.473178 |
Target: 5'- cGCGUUCGACaccgcuaCCGGUGCUGucgaUgCGa -3' miRNA: 3'- uCGCAAGCUG-------GGCCACGACug--AgGCc -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 31709 | 0.68 | 0.464313 |
Target: 5'- aAGCGUUCGuCaaaGGcaGCuUGGCUCCGGu -3' miRNA: 3'- -UCGCAAGCuGgg-CCa-CG-ACUGAGGCC- -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 21621 | 0.68 | 0.454565 |
Target: 5'- cGCG-UCGGCacauCGGUGCUcGGCUacCCGGu -3' miRNA: 3'- uCGCaAGCUGg---GCCACGA-CUGA--GGCC- -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 33400 | 0.68 | 0.444929 |
Target: 5'- gAGCGgugUCGACCgGcGcGCUGGCgaaCGGg -3' miRNA: 3'- -UCGCa--AGCUGGgC-CaCGACUGag-GCC- -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 17878 | 0.69 | 0.426007 |
Target: 5'- aAGCGUUCGGCCagaucguaGGUGCggucauucaguUGGCcCCGa -3' miRNA: 3'- -UCGCAAGCUGGg-------CCACG-----------ACUGaGGCc -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 43918 | 0.77 | 0.130613 |
Target: 5'- aGGUGUagcUCGuCCCGGUGCUGAgaucgagcgucUUCCGGu -3' miRNA: 3'- -UCGCA---AGCuGGGCCACGACU-----------GAGGCC- -5' |
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7322 | 5' | -57.8 | NC_001900.1 | + | 24494 | 1.1 | 0.000533 |
Target: 5'- cAGCGUUCGACCCGGUGCUGACUCCGGu -3' miRNA: 3'- -UCGCAAGCUGGGCCACGACUGAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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