Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7324 | 5' | -51.8 | NC_001900.1 | + | 48913 | 0.66 | 0.842747 |
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Target: 5'- cGGCAUUCGCAucGUC-GCAGGUCa---- -3' miRNA: 3'- -UCGUAGGCGU--UAGaCGUCUAGcagau -5' |
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| 7324 | 5' | -51.8 | NC_001900.1 | + | 33688 | 0.66 | 0.833464 |
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Target: 5'- uGUcgCCGcCGAUCUGCAGAgguUC-UCUGg -3' miRNA: 3'- uCGuaGGC-GUUAGACGUCU---AGcAGAU- -5' |
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| 7324 | 5' | -51.8 | NC_001900.1 | + | 31249 | 0.67 | 0.77325 |
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Target: 5'- gGGCAgCCaGCAGguugugCUGcCAGGUCGUCa- -3' miRNA: 3'- -UCGUaGG-CGUUa-----GAC-GUCUAGCAGau -5' |
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| 7324 | 5' | -51.8 | NC_001900.1 | + | 5974 | 0.69 | 0.672957 |
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Target: 5'- gAGcCGUCCGgGuUCUGCGGGUCGcCg- -3' miRNA: 3'- -UC-GUAGGCgUuAGACGUCUAGCaGau -5' |
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| 7324 | 5' | -51.8 | NC_001900.1 | + | 15912 | 0.7 | 0.569071 |
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Target: 5'- cGC-UCCGC-AUUUGCgAGAUCGUCg- -3' miRNA: 3'- uCGuAGGCGuUAGACG-UCUAGCAGau -5' |
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| 7324 | 5' | -51.8 | NC_001900.1 | + | 21899 | 0.71 | 0.557676 |
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Target: 5'- gGGCGcUCCGCAAUCucccguugauUGCAGG-CGUCg- -3' miRNA: 3'- -UCGU-AGGCGUUAG----------ACGUCUaGCAGau -5' |
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| 7324 | 5' | -51.8 | NC_001900.1 | + | 27669 | 0.74 | 0.389107 |
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Target: 5'- uGCAUCCGgGAUCgaGCGcGUCGUCUAc -3' miRNA: 3'- uCGUAGGCgUUAGa-CGUcUAGCAGAU- -5' |
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| 7324 | 5' | -51.8 | NC_001900.1 | + | 25216 | 1.07 | 0.002186 |
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Target: 5'- cAGCAUCCGCAAUCUGCAGAUCGUCUAc -3' miRNA: 3'- -UCGUAGGCGUUAGACGUCUAGCAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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