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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 7326 | 3' | -60.9 | NC_001900.1 | + | 26790 | 1.06 | 0.000301 |
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Target: 5'- aAAGCCCCCCGCCUGCUGCAACAGACGa -3' miRNA: 3'- -UUCGGGGGGCGGACGACGUUGUCUGC- -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 26871 | 0.89 | 0.00635 |
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Target: 5'- -cGCCCCUCGUCUGUUGCAGCAGGCGg -3' miRNA: 3'- uuCGGGGGGCGGACGACGUUGUCUGC- -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 5075 | 0.71 | 0.165639 |
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Target: 5'- cGGCCCCcgCCGCCgUGCUGgAGCccGACGa -3' miRNA: 3'- uUCGGGG--GGCGG-ACGACgUUGu-CUGC- -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 3002 | 0.7 | 0.179563 |
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Target: 5'- -uGCUCUCCGCaCUGCUGCuGACcgaAGGCGa -3' miRNA: 3'- uuCGGGGGGCG-GACGACG-UUG---UCUGC- -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 46369 | 0.7 | 0.189414 |
|
Target: 5'- gAAGCCUCUgGCCgcuggcGUUGUAGCAGGCa -3' miRNA: 3'- -UUCGGGGGgCGGa-----CGACGUUGUCUGc -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 24180 | 0.68 | 0.272342 |
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Target: 5'- -cGgCUCCCGCCgUGCUGaCGAacaAGACGa -3' miRNA: 3'- uuCgGGGGGCGG-ACGAC-GUUg--UCUGC- -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 33015 | 0.67 | 0.279264 |
|
Target: 5'- gGGGCUUCCugCGCCUGCUGCGgaGCccgAGACu -3' miRNA: 3'- -UUCGGGGG--GCGGACGACGU--UG---UCUGc -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 16947 | 0.67 | 0.308352 |
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Target: 5'- uGGCCUgCCGUcgCUGCUGUAGCGuucGGCGc -3' miRNA: 3'- uUCGGGgGGCG--GACGACGUUGU---CUGC- -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 20924 | 0.66 | 0.364682 |
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Target: 5'- cAGCCaUCCCGaagugggaUgaGCUGCAACAGACc -3' miRNA: 3'- uUCGG-GGGGC--------GgaCGACGUUGUCUGc -5' |
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| 7326 | 3' | -60.9 | NC_001900.1 | + | 21987 | 0.66 | 0.329262 |
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Target: 5'- -cGCCCCgaCGCCUGCaaucaacgggagauUGCGGagcgccCAGACGa -3' miRNA: 3'- uuCGGGGg-GCGGACG--------------ACGUU------GUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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