Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7326 | 5' | -55.6 | NC_001900.1 | + | 1248 | 0.67 | 0.533435 |
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Target: 5'- gGCGgCGGcagCGGAGCUGGcgGCGGg- -3' miRNA: 3'- aUGUgGUCua-GCCUCGACCa-CGUCac -5' |
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| 7326 | 5' | -55.6 | NC_001900.1 | + | 21217 | 0.68 | 0.490565 |
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Target: 5'- -cCACCGGccaGGaAGCUGGUGuCGGUGc -3' miRNA: 3'- auGUGGUCuagCC-UCGACCAC-GUCAC- -5' |
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| 7326 | 5' | -55.6 | NC_001900.1 | + | 46855 | 0.69 | 0.439366 |
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Target: 5'- aACGCCaagGGAUCGGccugcGCUaGGUGCGGUc -3' miRNA: 3'- aUGUGG---UCUAGCCu----CGA-CCACGUCAc -5' |
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| 7326 | 5' | -55.6 | NC_001900.1 | + | 33716 | 0.74 | 0.229699 |
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Target: 5'- aGCGCCuGGAacgccaGGAGCUGGUGCAGc- -3' miRNA: 3'- aUGUGG-UCUag----CCUCGACCACGUCac -5' |
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| 7326 | 5' | -55.6 | NC_001900.1 | + | 25973 | 0.75 | 0.200619 |
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Target: 5'- cACACCGGA--GGAGCUGGacGUAGUGg -3' miRNA: 3'- aUGUGGUCUagCCUCGACCa-CGUCAC- -5' |
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| 7326 | 5' | -55.6 | NC_001900.1 | + | 26824 | 1.07 | 0.000876 |
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Target: 5'- gUACACCAGAUCGGAGCUGGUGCAGUGa -3' miRNA: 3'- -AUGUGGUCUAGCCUCGACCACGUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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