Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7327 | 3' | -58.8 | NC_001900.1 | + | 20502 | 0.66 | 0.450299 |
Target: 5'- -cGGGCCG-GCCAagugguGCUUGCUGgUCa -3' miRNA: 3'- uaCUCGGCuCGGUgu----CGAACGAC-GGc -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 18743 | 0.66 | 0.440443 |
Target: 5'- -cGAGCUGcGCCuGCAGCgccGCacgGCCGg -3' miRNA: 3'- uaCUCGGCuCGG-UGUCGaa-CGa--CGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 2445 | 0.66 | 0.440443 |
Target: 5'- uGUGAgGUCGAcgGCCcCGGCUgcguccggGCUGCCa -3' miRNA: 3'- -UACU-CGGCU--CGGuGUCGAa-------CGACGGc -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 36391 | 0.66 | 0.430711 |
Target: 5'- uUG-GCCGAGCCcgACAGgaUGUUGUgGa -3' miRNA: 3'- uACuCGGCUCGG--UGUCgaACGACGgC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 18438 | 0.66 | 0.421105 |
Target: 5'- --aAGCCG-GUCACAGCguucuucacGCUGCUGg -3' miRNA: 3'- uacUCGGCuCGGUGUCGaa-------CGACGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 18229 | 0.66 | 0.41163 |
Target: 5'- -gGAGCCGAcCCACucGGCgacUGC-GCCGa -3' miRNA: 3'- uaCUCGGCUcGGUG--UCGa--ACGaCGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 34875 | 0.67 | 0.402289 |
Target: 5'- -aGAgGCCGuGCCGCcgaacgucuucAGCUUGCgacccGCCGc -3' miRNA: 3'- uaCU-CGGCuCGGUG-----------UCGAACGa----CGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 1824 | 0.67 | 0.402289 |
Target: 5'- -cGGGCuUGAGgUAgAGCUuggUGCUGCCGu -3' miRNA: 3'- uaCUCG-GCUCgGUgUCGA---ACGACGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 6324 | 0.67 | 0.393083 |
Target: 5'- -cGcAGCCGcaccGGCCACGGCc-GCUGUCa -3' miRNA: 3'- uaC-UCGGC----UCGGUGUCGaaCGACGGc -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 31624 | 0.67 | 0.3663 |
Target: 5'- -cGGGCCGA-CCGgAGCcaaGCUGCCu -3' miRNA: 3'- uaCUCGGCUcGGUgUCGaa-CGACGGc -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 25552 | 0.67 | 0.3663 |
Target: 5'- -cGGGCCguggGAGCCAC-GCUggcgGCUcagGCCGg -3' miRNA: 3'- uaCUCGG----CUCGGUGuCGAa---CGA---CGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 15781 | 0.68 | 0.349156 |
Target: 5'- -cGAGCUGaAGCCGCuGCUgaaGCUggGCCa -3' miRNA: 3'- uaCUCGGC-UCGGUGuCGAa--CGA--CGGc -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 33227 | 0.68 | 0.335858 |
Target: 5'- cGUGAcGCUGgcguacuucggcaccAGCCACGGCgcggaguaGCUGCCGu -3' miRNA: 3'- -UACU-CGGC---------------UCGGUGUCGaa------CGACGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 33067 | 0.69 | 0.293739 |
Target: 5'- ---cGCCGcuaccGCCGCcaccggaguucGGCUUGCUGCCGc -3' miRNA: 3'- uacuCGGCu----CGGUG-----------UCGAACGACGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 27042 | 0.71 | 0.220896 |
Target: 5'- cUGAGCCGAcacgguugguacGCCugGGC--GCUGCUGa -3' miRNA: 3'- uACUCGGCU------------CGGugUCGaaCGACGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 27468 | 0.72 | 0.164025 |
Target: 5'- -gGGGCCGAGCCGCAcgGCcUUGa-GCCGg -3' miRNA: 3'- uaCUCGGCUCGGUGU--CG-AACgaCGGC- -5' |
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7327 | 3' | -58.8 | NC_001900.1 | + | 28327 | 1.06 | 0.000472 |
Target: 5'- aAUGAGCCGAGCCACAGCUUGCUGCCGu -3' miRNA: 3'- -UACUCGGCUCGGUGUCGAACGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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