Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7328 | 3' | -54.1 | NC_001900.1 | + | 5213 | 0.66 | 0.808202 |
Target: 5'- gCGCgugCGCCGUCGUagaGGaacacccccacgccgUUGAcuUCUCCUc -3' miRNA: 3'- gGUGa--GCGGCAGCA---CC---------------AACU--AGAGGG- -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 29804 | 0.66 | 0.775063 |
Target: 5'- uCCGCUCcggGUCGUCcuUGGUgaugUGGUC-CCCg -3' miRNA: 3'- -GGUGAG---CGGCAGc-ACCA----ACUAGaGGG- -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 17976 | 0.66 | 0.775063 |
Target: 5'- -aGCUCGCucCGUCGcUGGUUcaGAUCgUCCa -3' miRNA: 3'- ggUGAGCG--GCAGC-ACCAA--CUAG-AGGg -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 19545 | 0.67 | 0.733934 |
Target: 5'- aCAC-CGCCGUggcCGUGGgagGAaCUgCCCa -3' miRNA: 3'- gGUGaGCGGCA---GCACCaa-CUaGA-GGG- -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 35995 | 0.67 | 0.723369 |
Target: 5'- aUCGCgauguUCGCCGUCG-GGUcagagUGGccCUCCCc -3' miRNA: 3'- -GGUG-----AGCGGCAGCaCCA-----ACUa-GAGGG- -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 44833 | 0.68 | 0.701979 |
Target: 5'- gCCAuUUCGCCGUUGccGGUcaacgcGGUCUCCa -3' miRNA: 3'- -GGU-GAGCGGCAGCa-CCAa-----CUAGAGGg -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 32601 | 0.68 | 0.701979 |
Target: 5'- cCCACgUCGagGaUGUGGUUGAgguagucgacguUCUCCCg -3' miRNA: 3'- -GGUG-AGCggCaGCACCAACU------------AGAGGG- -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 34728 | 0.68 | 0.647512 |
Target: 5'- aCACUgCGCgauggCGUGGUcGAUCUCCa -3' miRNA: 3'- gGUGA-GCGgca--GCACCAaCUAGAGGg -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 24983 | 0.7 | 0.528045 |
Target: 5'- gCGC-CGCCGUCGUaGGUccGGUCUCg- -3' miRNA: 3'- gGUGaGCGGCAGCA-CCAa-CUAGAGgg -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 22997 | 0.72 | 0.446424 |
Target: 5'- gCGCUCGucagaCCGUUGaccagagccUGGggGAUCUCCCc -3' miRNA: 3'- gGUGAGC-----GGCAGC---------ACCaaCUAGAGGG- -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 36527 | 0.73 | 0.390052 |
Target: 5'- ----cCGCUGUCa-GGUUGGUCUCCCa -3' miRNA: 3'- ggugaGCGGCAGcaCCAACUAGAGGG- -5' |
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7328 | 3' | -54.1 | NC_001900.1 | + | 28575 | 1.15 | 0.000581 |
Target: 5'- cCCACUCGCCGUCGUGGUUGAUCUCCCa -3' miRNA: 3'- -GGUGAGCGGCAGCACCAACUAGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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