Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7328 | 5' | -52.6 | NC_001900.1 | + | 25452 | 0.66 | 0.787167 |
Target: 5'- -aGAugGCCuCAgcagCCcgUGACGAGGAGa -3' miRNA: 3'- aaCUugCGGuGUa---GGa-GCUGUUCCUU- -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 33789 | 0.66 | 0.787167 |
Target: 5'- -cGAACcgugcgGCCACggCCUCGAcCAAGGu- -3' miRNA: 3'- aaCUUG------CGGUGuaGGAGCU-GUUCCuu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 27085 | 0.66 | 0.776724 |
Target: 5'- -cGGuCGCCAUcgUCgCGACGAGGAu -3' miRNA: 3'- aaCUuGCGGUGuaGGaGCUGUUCCUu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 44404 | 0.66 | 0.755356 |
Target: 5'- --cGACGCCGuCGUCguggUCGACGGGGAGc -3' miRNA: 3'- aacUUGCGGU-GUAGg---AGCUGUUCCUU- -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 1406 | 0.66 | 0.755356 |
Target: 5'- gUUGAACGCCGCAcgCUUCuGCGcGGGGu -3' miRNA: 3'- -AACUUGCGGUGUa-GGAGcUGUuCCUU- -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 1184 | 0.67 | 0.733433 |
Target: 5'- -cGggUGCUguuGCGUCCUCGGguucCAGGGGc -3' miRNA: 3'- aaCuuGCGG---UGUAGGAGCU----GUUCCUu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 148 | 0.67 | 0.711061 |
Target: 5'- cUGGugGCuCACAgcgCCUgGGCGAGGc- -3' miRNA: 3'- aACUugCG-GUGUa--GGAgCUGUUCCuu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 1118 | 0.67 | 0.69974 |
Target: 5'- -aGGACGCaacaGCA-CC-CGGCAAGGAGc -3' miRNA: 3'- aaCUUGCGg---UGUaGGaGCUGUUCCUU- -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 20384 | 0.67 | 0.688345 |
Target: 5'- -cGAGCGCCuuGC-UUCUCGACGuugAGGAu -3' miRNA: 3'- aaCUUGCGG--UGuAGGAGCUGU---UCCUu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 15973 | 0.68 | 0.676892 |
Target: 5'- cUGAuCGCCGCG--CUgGACGAGGAAg -3' miRNA: 3'- aACUuGCGGUGUagGAgCUGUUCCUU- -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 13023 | 0.68 | 0.665391 |
Target: 5'- -gGAACGCgUACucagCCUCGACAcGGAc -3' miRNA: 3'- aaCUUGCG-GUGua--GGAGCUGUuCCUu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 8916 | 0.69 | 0.573135 |
Target: 5'- gUGAGCGCCugGaggacgcggUCCUCGaagGCGAGGu- -3' miRNA: 3'- aACUUGCGGugU---------AGGAGC---UGUUCCuu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 3600 | 0.7 | 0.539135 |
Target: 5'- -cGAGCGCCGCGUCgaUGGCGuccuGGAu -3' miRNA: 3'- aaCUUGCGGUGUAGgaGCUGUu---CCUu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 32685 | 0.73 | 0.391776 |
Target: 5'- -aGAACGucgacuaccucaaCCACAUCCUCGACGuGGGc -3' miRNA: 3'- aaCUUGC-------------GGUGUAGGAGCUGUuCCUu -5' |
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7328 | 5' | -52.6 | NC_001900.1 | + | 28609 | 1.04 | 0.002779 |
Target: 5'- cUUGAACGCCACAUCCUCGACAAGGAAa -3' miRNA: 3'- -AACUUGCGGUGUAGGAGCUGUUCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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