Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7331 | 3' | -54.1 | NC_001900.1 | + | 28893 | 1.1 | 0.001108 |
Target: 5'- uUGCCGUCGGGAGUCCGAACACGAAACu -3' miRNA: 3'- -ACGGCAGCCCUCAGGCUUGUGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 9655 | 0.74 | 0.308941 |
Target: 5'- aUGCgCGUCGGGGGcuccacCCGGAUcaGCGGGACg -3' miRNA: 3'- -ACG-GCAGCCCUCa-----GGCUUG--UGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 28014 | 0.72 | 0.402773 |
Target: 5'- cGCCGuUCGGGccGGUguaaCCGggUGCGGAGCc -3' miRNA: 3'- aCGGC-AGCCC--UCA----GGCuuGUGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 35005 | 0.7 | 0.534139 |
Target: 5'- aGCCcucgCGGGAGUCCucGAuCACGAAcGCa -3' miRNA: 3'- aCGGca--GCCCUCAGG--CUuGUGCUU-UG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 3872 | 0.7 | 0.534139 |
Target: 5'- cGCCGUCGaGGucacgCCGAcCGCGAAGg -3' miRNA: 3'- aCGGCAGC-CCuca--GGCUuGUGCUUUg -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 31274 | 0.69 | 0.554727 |
Target: 5'- gGUCGUCaGGAGUCCGAucucgccAC-CGAAGu -3' miRNA: 3'- aCGGCAGcCCUCAGGCU-------UGuGCUUUg -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 20191 | 0.68 | 0.655452 |
Target: 5'- cUGCUGgagUGGGAGUucaUCGAGaACGAGACc -3' miRNA: 3'- -ACGGCa--GCCCUCA---GGCUUgUGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 41953 | 0.67 | 0.677609 |
Target: 5'- aGaCGUUGaGcGAGUCCGGGC-CGAAGCc -3' miRNA: 3'- aCgGCAGC-C-CUCAGGCUUGuGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 11043 | 0.67 | 0.688627 |
Target: 5'- gGCUGggCGaGGGUcgccaCCGGGCGCGAAACa -3' miRNA: 3'- aCGGCa-GCcCUCA-----GGCUUGUGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 30952 | 0.67 | 0.710485 |
Target: 5'- aUGCUGgggCGGGAGgagguGCAUGAGGCg -3' miRNA: 3'- -ACGGCa--GCCCUCaggcuUGUGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 20062 | 0.67 | 0.718066 |
Target: 5'- gGCC-UCGGGAG-CCGAAgGguaaccacuuguccCGGAGCg -3' miRNA: 3'- aCGGcAGCCCUCaGGCUUgU--------------GCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 24979 | 0.67 | 0.721302 |
Target: 5'- cGCCGUCGuaGGUCCGGucuCGguACa -3' miRNA: 3'- aCGGCAGCccUCAGGCUuguGCuuUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 8606 | 0.66 | 0.732029 |
Target: 5'- aGCCcaucuuGUCGaGGuacuuGUCCGggUcgGCGAAGCa -3' miRNA: 3'- aCGG------CAGC-CCu----CAGGCuuG--UGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 45148 | 0.66 | 0.732029 |
Target: 5'- cUGCCG-CGuaccGGAGcCCGcugugccgaGACACGAAGCc -3' miRNA: 3'- -ACGGCaGC----CCUCaGGC---------UUGUGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 30190 | 0.66 | 0.742653 |
Target: 5'- cGCaCGUCGuGGGUCCGAcguuccuggacACuCGGGACu -3' miRNA: 3'- aCG-GCAGCcCUCAGGCU-----------UGuGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 9886 | 0.66 | 0.760445 |
Target: 5'- gUGCCGUacagguccgagagcCGGGuccGGUUCGGAaGCGGGACc -3' miRNA: 3'- -ACGGCA--------------GCCC---UCAGGCUUgUGCUUUG- -5' |
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7331 | 3' | -54.1 | NC_001900.1 | + | 40978 | 0.66 | 0.773792 |
Target: 5'- cGCgaaGUUGGucGAGUCCGAGgACGGGcuGCa -3' miRNA: 3'- aCGg--CAGCC--CUCAGGCUUgUGCUU--UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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