Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7332 | 5' | -57.5 | NC_001900.1 | + | 28948 | 0.66 | 0.561265 |
Target: 5'- -gGCC-AUCAGCGCCG-ACCCAg--- -3' miRNA: 3'- caCGGcUAGUCGUGGCcUGGGUagag -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 48121 | 0.66 | 0.550467 |
Target: 5'- aGUGuCCGcacuuuAUCGGCAUCGGACuuCCGcCUCa -3' miRNA: 3'- -CAC-GGC------UAGUCGUGGCCUG--GGUaGAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 12233 | 0.66 | 0.539733 |
Target: 5'- gGUGCCGAUgGGUACCacGGGCCagaagAUCcCg -3' miRNA: 3'- -CACGGCUAgUCGUGG--CCUGGg----UAGaG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 10989 | 0.66 | 0.517434 |
Target: 5'- -cGCCGAcagaucaUCGGCGCUGucaagaacGACCCAgCUCc -3' miRNA: 3'- caCGGCU-------AGUCGUGGC--------CUGGGUaGAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 9780 | 0.66 | 0.515329 |
Target: 5'- -gGCgCGGUCccGGUggucccgcuuccgaACCGGACCCGgcUCUCg -3' miRNA: 3'- caCG-GCUAG--UCG--------------UGGCCUGGGU--AGAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 3853 | 0.66 | 0.507989 |
Target: 5'- -gGCUGAUCGGCGCgGuGAacaCCGUCa- -3' miRNA: 3'- caCGGCUAGUCGUGgC-CUg--GGUAGag -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 39654 | 0.68 | 0.42771 |
Target: 5'- -aGCgCGAaCAGCACCGGGuuCAUC-Cg -3' miRNA: 3'- caCG-GCUaGUCGUGGCCUggGUAGaG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 43877 | 0.71 | 0.277824 |
Target: 5'- gGUGCCacuUCGGCAugucugggaUCGGACCCAgCUCa -3' miRNA: 3'- -CACGGcu-AGUCGU---------GGCCUGGGUaGAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 29364 | 1.08 | 0.000568 |
Target: 5'- gGUGCCGAUCAGCACCGGACCCAUCUCc -3' miRNA: 3'- -CACGGCUAGUCGUGGCCUGGGUAGAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 29427 | 0.73 | 0.208725 |
Target: 5'- gGUGCUGAUCGGCACC-GACCCc---- -3' miRNA: 3'- -CACGGCUAGUCGUGGcCUGGGuagag -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 14041 | 0.67 | 0.497582 |
Target: 5'- cGUGCCGAc--GUACCGGACUgGUUg- -3' miRNA: 3'- -CACGGCUaguCGUGGCCUGGgUAGag -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 23883 | 0.71 | 0.284937 |
Target: 5'- -cGCCGAU-AGCcgACCGGAgCCCGUCgUCg -3' miRNA: 3'- caCGGCUAgUCG--UGGCCU-GGGUAG-AG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 10000 | 0.7 | 0.307137 |
Target: 5'- aUGCCGAagaUCAGuCGCUGGggcacGCCCAUCa- -3' miRNA: 3'- cACGGCU---AGUC-GUGGCC-----UGGGUAGag -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 3912 | 0.7 | 0.322658 |
Target: 5'- -cGCCGAUCAGC-CCGGAgaagCCGUUg- -3' miRNA: 3'- caCGGCUAGUCGuGGCCUg---GGUAGag -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 3134 | 0.68 | 0.418206 |
Target: 5'- -cGCCGGUCGGgucgGCgGGugCCcgCUCg -3' miRNA: 3'- caCGGCUAGUCg---UGgCCugGGuaGAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 7867 | 0.68 | 0.42771 |
Target: 5'- uUGCCGAUC---GCCuGACCgGUCUCa -3' miRNA: 3'- cACGGCUAGucgUGGcCUGGgUAGAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 9031 | 0.68 | 0.447096 |
Target: 5'- cUGCCGA--AGCACCGGGuugCCGUCg- -3' miRNA: 3'- cACGGCUagUCGUGGCCUg--GGUAGag -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 43940 | 0.67 | 0.45697 |
Target: 5'- aUGCCGAagUGGCACCagucGGCCCAgccCUCg -3' miRNA: 3'- cACGGCUa-GUCGUGGc---CUGGGUa--GAG- -5' |
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7332 | 5' | -57.5 | NC_001900.1 | + | 10570 | 0.67 | 0.487271 |
Target: 5'- cGUGCC---CAGCagGCCGaGGCCCAUCg- -3' miRNA: 3'- -CACGGcuaGUCG--UGGC-CUGGGUAGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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