Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7333 | 5' | -58.3 | NC_001900.1 | + | 40423 | 0.66 | 0.553767 |
Target: 5'- uCGCCGAGcACGgguUCCGAGucaucgcacucgacGCGG-CCAa -3' miRNA: 3'- -GCGGCUCaUGC---AGGCUCu-------------CGCCaGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 21403 | 0.66 | 0.539071 |
Target: 5'- gCGCCGAacACGUCgGAguaGAGCgGGUUCAg -3' miRNA: 3'- -GCGGCUcaUGCAGgCU---CUCG-CCAGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 8597 | 0.66 | 0.528656 |
Target: 5'- uGUCGAGguacuUGUCCGGGucggcgaagcAGCGGUuCCAc -3' miRNA: 3'- gCGGCUCau---GCAGGCUC----------UCGCCA-GGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 1186 | 0.66 | 0.518316 |
Target: 5'- cCGCCGGaaGCGgCCG-GGGCGGUUUAg -3' miRNA: 3'- -GCGGCUcaUGCaGGCuCUCGCCAGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 15141 | 0.67 | 0.512151 |
Target: 5'- aGCCGAGgcACGcuccccgaguucggcUUCGAGGGCGGcgauUCCGa -3' miRNA: 3'- gCGGCUCa-UGC---------------AGGCUCUCGCC----AGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 18162 | 0.67 | 0.508057 |
Target: 5'- uCGCCGAGUGgGUCgGcuccUGGUCCAg -3' miRNA: 3'- -GCGGCUCAUgCAGgCucucGCCAGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 14361 | 0.67 | 0.497885 |
Target: 5'- gGCCaGGUugGUUCGAGcacGGCGGgugacgCCGu -3' miRNA: 3'- gCGGcUCAugCAGGCUC---UCGCCa-----GGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 26378 | 0.67 | 0.487805 |
Target: 5'- aCGCCGAagcGUggcucGCGUCUGAGAaGCGG-CUg -3' miRNA: 3'- -GCGGCU---CA-----UGCAGGCUCU-CGCCaGGu -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 38838 | 0.68 | 0.411016 |
Target: 5'- cCGCCGAGUAU-UUgGAGAgucGCGGUCUu -3' miRNA: 3'- -GCGGCUCAUGcAGgCUCU---CGCCAGGu -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 5734 | 0.68 | 0.401953 |
Target: 5'- uCGCUGAGUGCGuugaugUCUGGGcGCucgacGGUCCAg -3' miRNA: 3'- -GCGGCUCAUGC------AGGCUCuCG-----CCAGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 36492 | 0.69 | 0.38421 |
Target: 5'- gGCCGuGaucUACGUCCGcaAGCaGGUCCAc -3' miRNA: 3'- gCGGCuC---AUGCAGGCucUCG-CCAGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 9885 | 0.73 | 0.197445 |
Target: 5'- uGCCGuacAgGUCCGAGAGCcgGGUCCGg -3' miRNA: 3'- gCGGCucaUgCAGGCUCUCG--CCAGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 10661 | 0.79 | 0.087342 |
Target: 5'- uCGCCGGGUACGUCC---AGCGGUUCGc -3' miRNA: 3'- -GCGGCUCAUGCAGGcucUCGCCAGGU- -5' |
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7333 | 5' | -58.3 | NC_001900.1 | + | 29567 | 1.08 | 0.000612 |
Target: 5'- gCGCCGAGUACGUCCGAGAGCGGUCCAc -3' miRNA: 3'- -GCGGCUCAUGCAGGCUCUCGCCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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