Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7334 | 5' | -55.2 | NC_001900.1 | + | 43864 | 0.66 | 0.714268 |
Target: 5'- gGCUGGGccGaCUGGUGccacuUCGGCAUGUc -3' miRNA: 3'- gCGGCCCuaC-GGUCACu----AGCUGUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 2432 | 0.66 | 0.670661 |
Target: 5'- uGCCGGGuggcUGUguGaGGUCGACG-GCc -3' miRNA: 3'- gCGGCCCu---ACGguCaCUAGCUGUaCG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 11606 | 0.67 | 0.65964 |
Target: 5'- gGCCGGaGUGCCuucgguaguugcGGUG-UCGgACAUGUg -3' miRNA: 3'- gCGGCCcUACGG------------UCACuAGC-UGUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 20972 | 0.67 | 0.648593 |
Target: 5'- gGCUGGaGAcgGCCAGgucgaaggGGUCGACGUa- -3' miRNA: 3'- gCGGCC-CUa-CGGUCa-------CUAGCUGUAcg -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 45094 | 0.67 | 0.604354 |
Target: 5'- uCGCCGaGGAgauucugaagGCCGaccaGGUUGACGUGCu -3' miRNA: 3'- -GCGGC-CCUa---------CGGUca--CUAGCUGUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 3080 | 0.67 | 0.604354 |
Target: 5'- cCGgCGGGAagGUCGuugacGUGAcCGACGUGCu -3' miRNA: 3'- -GCgGCCCUa-CGGU-----CACUaGCUGUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 6843 | 0.68 | 0.593331 |
Target: 5'- uCGCaGGGA-GCCAucGUGGUUGGCcagGUGCu -3' miRNA: 3'- -GCGgCCCUaCGGU--CACUAGCUG---UACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 11253 | 0.68 | 0.593331 |
Target: 5'- uGCCuGGuGGUGCCuGUcuUCGACGUGg -3' miRNA: 3'- gCGG-CC-CUACGGuCAcuAGCUGUACg -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 3504 | 0.68 | 0.59003 |
Target: 5'- cCGCaGGGucugGCCGGUGAcauccaggacgccaUCGACGcgGCg -3' miRNA: 3'- -GCGgCCCua--CGGUCACU--------------AGCUGUa-CG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 23992 | 0.68 | 0.571392 |
Target: 5'- cCGCUGGGGucguUGUCGuUGAUCGugAcGCg -3' miRNA: 3'- -GCGGCCCU----ACGGUcACUAGCugUaCG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 24298 | 0.69 | 0.507022 |
Target: 5'- uGCCacuGGGAUGUCGGUGA---GCGUGUu -3' miRNA: 3'- gCGG---CCCUACGGUCACUagcUGUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 14702 | 0.69 | 0.496579 |
Target: 5'- aCGCCccGAagcugcacucgcUGCCGGUGGUCGAgAUGUc -3' miRNA: 3'- -GCGGccCU------------ACGGUCACUAGCUgUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 30732 | 0.69 | 0.482126 |
Target: 5'- gGCCGGGGguccaccagcuucGCCAG-GAUCuGCGUGUc -3' miRNA: 3'- gCGGCCCUa------------CGGUCaCUAGcUGUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 47641 | 0.73 | 0.313416 |
Target: 5'- uGCCuugcGGAUgacggugucGCCAGUGAUCGugAUGUc -3' miRNA: 3'- gCGGc---CCUA---------CGGUCACUAGCugUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 3524 | 0.75 | 0.223956 |
Target: 5'- gGCCGGGGUGUCGGUGccGUUGAgguucaggguguaCGUGCc -3' miRNA: 3'- gCGGCCCUACGGUCAC--UAGCU-------------GUACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 8225 | 0.77 | 0.166948 |
Target: 5'- uGCCGGGAUGUgGGcguugcaGAUCGACAgggUGCg -3' miRNA: 3'- gCGGCCCUACGgUCa------CUAGCUGU---ACG- -5' |
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7334 | 5' | -55.2 | NC_001900.1 | + | 30025 | 1.13 | 0.000517 |
Target: 5'- gCGCCGGGAUGCCAGUGAUCGACAUGCg -3' miRNA: 3'- -GCGGCCCUACGGUCACUAGCUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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