Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7335 | 5' | -55.2 | NC_001900.1 | + | 22016 | 0.66 | 0.669379 |
Target: 5'- gGAGCUgGCUGaUGAGgacgGCaacaUCCACGUCa- -3' miRNA: 3'- -UUCGA-CGAC-GCUCa---UG----AGGUGCAGcu -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 8356 | 0.68 | 0.513764 |
Target: 5'- gGGGCUGUUGaucgccCGAGgcgACUCCACGUgGc -3' miRNA: 3'- -UUCGACGAC------GCUCa--UGAGGUGCAgCu -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 17277 | 0.7 | 0.393118 |
Target: 5'- gGAGCUGCUG-GAGccccggacccUGCgacaCCACGUCGGu -3' miRNA: 3'- -UUCGACGACgCUC----------AUGa---GGUGCAGCU- -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 33949 | 0.7 | 0.393118 |
Target: 5'- -cGCgGCUGaCGGGUA--CCACGUCGAg -3' miRNA: 3'- uuCGaCGAC-GCUCAUgaGGUGCAGCU- -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 40314 | 0.71 | 0.374852 |
Target: 5'- uGAGCcGCUGCGgccguGGUuggCCGCGUCGAg -3' miRNA: 3'- -UUCGaCGACGC-----UCAugaGGUGCAGCU- -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 11373 | 0.72 | 0.312408 |
Target: 5'- aGAGCUGCagagcgcguuccugUGCGgccuuuccgggccAGUuCUCCACGUCGAa -3' miRNA: 3'- -UUCGACG--------------ACGC-------------UCAuGAGGUGCAGCU- -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 26301 | 0.75 | 0.201769 |
Target: 5'- aAGGCUGCgGCGAGUGCcaUCACgGUCGAg -3' miRNA: 3'- -UUCGACGaCGCUCAUGa-GGUG-CAGCU- -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 6031 | 0.8 | 0.088595 |
Target: 5'- cGAGCUGgaGCGAGUugUCCGCGcCGc -3' miRNA: 3'- -UUCGACgaCGCUCAugAGGUGCaGCu -5' |
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7335 | 5' | -55.2 | NC_001900.1 | + | 30391 | 1.07 | 0.001063 |
Target: 5'- cAAGCUGCUGCGAGUACUCCACGUCGAg -3' miRNA: 3'- -UUCGACGACGCUCAUGAGGUGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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