Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7336 | 3' | -54.4 | NC_001900.1 | + | 44797 | 0.66 | 0.722379 |
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Target: 5'- ----cGCUCGgaucgacaaggugugGCaGCgGCCAGCGGCCu -3' miRNA: 3'- gaacaCGAGCa--------------UGcCG-UGGUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 7928 | 0.66 | 0.710485 |
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Target: 5'- --cGUGUucuUCGUcUGGUccugGCUGACGGCCg -3' miRNA: 3'- gaaCACG---AGCAuGCCG----UGGUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 27567 | 0.67 | 0.688627 |
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Target: 5'- --gGUGCgcCGcUACauCGCCGACGGCCg -3' miRNA: 3'- gaaCACGa-GC-AUGccGUGGUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 46455 | 0.67 | 0.677609 |
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Target: 5'- aCUgcgGUGCcUGcUACaaCGCCAGCGGCCa -3' miRNA: 3'- -GAa--CACGaGC-AUGccGUGGUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 31691 | 0.67 | 0.666547 |
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Target: 5'- gCUUG-GCUCcgguCGGC-CCGAacCGGCCg -3' miRNA: 3'- -GAACaCGAGcau-GCCGuGGUU--GCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 1614 | 0.68 | 0.644335 |
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Target: 5'- --gGUGCuUCGUGCGGUggACCAccuGC-GCCu -3' miRNA: 3'- gaaCACG-AGCAUGCCG--UGGU---UGcCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 28055 | 0.68 | 0.610956 |
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Target: 5'- --cGUGCccgcCGgggGCGGCGUCAACGuGCCg -3' miRNA: 3'- gaaCACGa---GCa--UGCCGUGGUUGC-CGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 23237 | 0.69 | 0.566754 |
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Target: 5'- ---cUGCUCGgcCGGCucaaCGACGGUCu -3' miRNA: 3'- gaacACGAGCauGCCGug--GUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 834 | 0.69 | 0.534139 |
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Target: 5'- cCUUGcgacUGC-CaGUACGcgccGCACCAGCGGCUg -3' miRNA: 3'- -GAAC----ACGaG-CAUGC----CGUGGUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 40831 | 0.66 | 0.753162 |
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Target: 5'- -gUGgugGUUCacgGCGGCGCUcGCGGCg -3' miRNA: 3'- gaACa--CGAGca-UGCCGUGGuUGCCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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