Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7336 | 3' | -54.4 | NC_001900.1 | + | 28055 | 0.68 | 0.610956 |
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Target: 5'- --cGUGCccgcCGgggGCGGCGUCAACGuGCCg -3' miRNA: 3'- gaaCACGa---GCa--UGCCGUGGUUGC-CGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 31869 | 0.68 | 0.588781 |
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Target: 5'- --gGUGCUUcuuCcGCAUCAGCGGCCu -3' miRNA: 3'- gaaCACGAGcauGcCGUGGUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 23237 | 0.69 | 0.566754 |
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Target: 5'- ---cUGCUCGgcCGGCucaaCGACGGUCu -3' miRNA: 3'- gaacACGAGCauGCCGug--GUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 28201 | 0.69 | 0.544943 |
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Target: 5'- cCUUGUGCgaugUCGU-UGGCcucguccuuGCgGACGGCCu -3' miRNA: 3'- -GAACACG----AGCAuGCCG---------UGgUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 834 | 0.69 | 0.534139 |
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Target: 5'- cCUUGcgacUGC-CaGUACGcgccGCACCAGCGGCUg -3' miRNA: 3'- -GAAC----ACGaG-CAUGC----CGUGGUUGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 23500 | 0.69 | 0.530913 |
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Target: 5'- ----cGCUCGUcguCGGCACCAuccccggugaggcuGCaGGCCu -3' miRNA: 3'- gaacaCGAGCAu--GCCGUGGU--------------UG-CCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 16987 | 0.7 | 0.471152 |
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Target: 5'- -gUGUGgUCGcacuCGGCAUgGACGGCa -3' miRNA: 3'- gaACACgAGCau--GCCGUGgUUGCCGg -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 4992 | 0.71 | 0.461015 |
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Target: 5'- ---aUGCUCGUcgggcuccagcACGGCGgCGGgGGCCg -3' miRNA: 3'- gaacACGAGCA-----------UGCCGUgGUUgCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 36721 | 0.74 | 0.286423 |
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Target: 5'- uCUUGUGCagGUACGGCAgcUUggUGGCUg -3' miRNA: 3'- -GAACACGagCAUGCCGU--GGuuGCCGG- -5' |
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| 7336 | 3' | -54.4 | NC_001900.1 | + | 30460 | 1.13 | 0.000624 |
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Target: 5'- uCUUGUGCUCGUACGGCACCAACGGCCg -3' miRNA: 3'- -GAACACGAGCAUGCCGUGGUUGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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