Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7336 | 5' | -62.3 | NC_001900.1 | + | 1224 | 0.67 | 0.283454 |
Target: 5'- gCaGGCACCGGUGGUGUacucgguggcggcggCAGCGgaGCUg -3' miRNA: 3'- aGcCCGUGGUCGCCACG---------------GUCGCg-UGA- -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 4000 | 0.72 | 0.112196 |
Target: 5'- gCGGGUACCGGCGucucgGCCAGCuCGCc -3' miRNA: 3'- aGCCCGUGGUCGCca---CGGUCGcGUGa -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 4998 | 0.68 | 0.229754 |
Target: 5'- gUCGGGCuCCagcacggcGGCGGggGCCGGUacucGCACa -3' miRNA: 3'- -AGCCCGuGG--------UCGCCa-CGGUCG----CGUGa -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 12240 | 0.69 | 0.212216 |
Target: 5'- aUGGGUACCA-CGG-GCCAGaagauccCGCACUg -3' miRNA: 3'- aGCCCGUGGUcGCCaCGGUC-------GCGUGA- -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 12552 | 0.66 | 0.332271 |
Target: 5'- aCGGGCugU--CGGUGCC-GUGCAUc -3' miRNA: 3'- aGCCCGugGucGCCACGGuCGCGUGa -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 12616 | 0.76 | 0.064342 |
Target: 5'- gUCGuGGCACCGGUGGUGUCAGCa---- -3' miRNA: 3'- -AGC-CCGUGGUCGCCACGGUCGcguga -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 19874 | 0.68 | 0.235667 |
Target: 5'- gUCGGGCuCCc-CGGUGUgGGCGCGg- -3' miRNA: 3'- -AGCCCGuGGucGCCACGgUCGCGUga -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 20500 | 0.66 | 0.332271 |
Target: 5'- uUCGGGCcgGCCaAGUGGUGCUuGCugguCACg -3' miRNA: 3'- -AGCCCG--UGG-UCGCCACGGuCGc---GUGa -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 21054 | 0.66 | 0.309346 |
Target: 5'- cUGGGCACCAGCccgaGG-GCUc-CGCACg -3' miRNA: 3'- aGCCCGUGGUCG----CCaCGGucGCGUGa -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 21949 | 0.66 | 0.316851 |
Target: 5'- cCGGcGUACUGGCGGauugGUCAGaGCACUu -3' miRNA: 3'- aGCC-CGUGGUCGCCa---CGGUCgCGUGA- -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 25553 | 0.67 | 0.273858 |
Target: 5'- cCGGGCcgugggagccacGCUGGCGGcucagGCCGGUGCGa- -3' miRNA: 3'- aGCCCG------------UGGUCGCCa----CGGUCGCGUga -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 30494 | 1.08 | 0.000198 |
Target: 5'- gUCGGGCACCAGCGGUGCCAGCGCACUg -3' miRNA: 3'- -AGCCCGUGGUCGCCACGGUCGCGUGA- -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 33127 | 0.71 | 0.135743 |
Target: 5'- gCGGuaGCGgCGGCGGUgGCCAGCaGCACc -3' miRNA: 3'- aGCC--CGUgGUCGCCA-CGGUCG-CGUGa -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 33369 | 0.67 | 0.254174 |
Target: 5'- gUCGGcgucugcugcuGCGCUucuucagcaggAGCGGUGucgaCCGGCGCGCUg -3' miRNA: 3'- -AGCC-----------CGUGG-----------UCGCCAC----GGUCGCGUGA- -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 39893 | 0.66 | 0.329923 |
Target: 5'- aUCGcGuGCAgCAGCGGUGUcgugucggcuucauCAGCGCGa- -3' miRNA: 3'- -AGC-C-CGUgGUCGCCACG--------------GUCGCGUga -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 40426 | 0.68 | 0.247875 |
Target: 5'- -aGGaaCACCAGCGGggcGCCGGUGC-CUa -3' miRNA: 3'- agCCc-GUGGUCGCCa--CGGUCGCGuGA- -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 41281 | 0.73 | 0.092514 |
Target: 5'- cCGcuGCACCAGCaGGUGCUGGgCGCGCUg -3' miRNA: 3'- aGCc-CGUGGUCG-CCACGGUC-GCGUGA- -5' |
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7336 | 5' | -62.3 | NC_001900.1 | + | 48567 | 0.7 | 0.163758 |
Target: 5'- -aGGGCACguGUGGcagGCCAGaCGCAa- -3' miRNA: 3'- agCCCGUGguCGCCa--CGGUC-GCGUga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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