Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7339 | 5' | -57.1 | NC_001900.1 | + | 47765 | 0.66 | 0.572121 |
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Target: 5'- --gGGCuGUUGUaGGUCCAGUcugGCGUCUg -3' miRNA: 3'- gugUCG-CAACAgCCGGGUCG---UGCAGA- -5' |
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| 7339 | 5' | -57.1 | NC_001900.1 | + | 12039 | 0.67 | 0.487271 |
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Target: 5'- aCACcGCGUacaacgcgaUGcagCGGUUCGGCACGUCg -3' miRNA: 3'- -GUGuCGCA---------ACa--GCCGGGUCGUGCAGa -5' |
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| 7339 | 5' | -57.1 | NC_001900.1 | + | 35075 | 0.67 | 0.477062 |
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Target: 5'- ----cCGUUGUCcuGCUCAGCACGUCg -3' miRNA: 3'- gugucGCAACAGc-CGGGUCGUGCAGa -5' |
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| 7339 | 5' | -57.1 | NC_001900.1 | + | 23311 | 0.71 | 0.307137 |
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Target: 5'- cCACGGCG--GUCGGCCCcGGCugGg-- -3' miRNA: 3'- -GUGUCGCaaCAGCCGGG-UCGugCaga -5' |
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| 7339 | 5' | -57.1 | NC_001900.1 | + | 39902 | 0.71 | 0.277824 |
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Target: 5'- aGCAGCGgugucgUGUCGGCUucauCAGCGCGa-- -3' miRNA: 3'- gUGUCGCa-----ACAGCCGG----GUCGUGCaga -5' |
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| 7339 | 5' | -57.1 | NC_001900.1 | + | 33684 | 1.07 | 0.0007 |
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Target: 5'- uCACAGCGUUGUCGGCCCAGCACGUCUg -3' miRNA: 3'- -GUGUCGCAACAGCCGGGUCGUGCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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