Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
734 | 3' | -55.7 | AC_000020.1 | + | 16000 | 0.66 | 0.505102 |
Target: 5'- gGGCUGGCAaaaaGCCUCCucucaugcGCCCGCa -3' miRNA: 3'- gUUGACUGUaac-CGGGGGuu------CGGGUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 10968 | 0.66 | 0.501809 |
Target: 5'- aGGCUGACucugcaUGGCCgCCAuuuucaugggguugAGCgCCGCg -3' miRNA: 3'- gUUGACUGua----ACCGGgGGU--------------UCG-GGUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 8305 | 0.66 | 0.494163 |
Target: 5'- cCGACUGuagaccaccuGCcccucgUGGUCCCU-AGCCCGCa -3' miRNA: 3'- -GUUGAC----------UGua----ACCGGGGGuUCGGGUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 4928 | 0.66 | 0.483334 |
Target: 5'- uGGCaUGACcuUUGGCCCUgAGcuugcccuuGCCCACg -3' miRNA: 3'- gUUG-ACUGu-AACCGGGGgUU---------CGGGUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 17062 | 0.66 | 0.47262 |
Target: 5'- -cACUG----UGGCCCCCA--CCCACg -3' miRNA: 3'- guUGACuguaACCGGGGGUucGGGUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 6023 | 0.66 | 0.47262 |
Target: 5'- ----gGACGUgcuGCCCCCAuuuuGCUCACg -3' miRNA: 3'- guugaCUGUAac-CGGGGGUu---CGGGUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 25751 | 0.66 | 0.46943 |
Target: 5'- -cGCUGGuauaaaguagacccCAgcGGCUUCCAAGCCCAa -3' miRNA: 3'- guUGACU--------------GUaaCCGGGGGUUCGGGUg -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 15544 | 0.69 | 0.311265 |
Target: 5'- ---aUGGCAUUggcgGGCCCCCAucuugaaGGCCgACg -3' miRNA: 3'- guugACUGUAA----CCGGGGGU-------UCGGgUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 15546 | 0.7 | 0.273451 |
Target: 5'- aCGGCUaGGaaaAUgagGGgCCCCAAGCCUACa -3' miRNA: 3'- -GUUGA-CUg--UAa--CCgGGGGUUCGGGUG- -5' |
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734 | 3' | -55.7 | AC_000020.1 | + | 25453 | 1.09 | 0.000338 |
Target: 5'- cCAACUGACAUUGGCCCCCAAGCCCACu -3' miRNA: 3'- -GUUGACUGUAACCGGGGGUUCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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