Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7341 | 3' | -54 | NC_001900.1 | + | 19972 | 0.66 | 0.751531 |
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Target: 5'- -aUGCGcUCCGGGacaagugguuACCcUUCGGCUCc -3' miRNA: 3'- caACGCuAGGUCU----------UGGaGAGCCGAGa -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 3821 | 0.66 | 0.740857 |
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Target: 5'- --gGCGGUCaAGAACUUCUucaaCGGCUUc -3' miRNA: 3'- caaCGCUAGgUCUUGGAGA----GCCGAGa -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 23081 | 0.66 | 0.730068 |
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Target: 5'- --cGCGAgCCAGGuaGCCUC-CgGGCUCc -3' miRNA: 3'- caaCGCUaGGUCU--UGGAGaG-CCGAGa -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 42597 | 0.68 | 0.629786 |
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Target: 5'- --aGCGGacUCCAGAGCCUgCUUGacgcGCUCg -3' miRNA: 3'- caaCGCU--AGGUCUUGGA-GAGC----CGAGa -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 40346 | 0.68 | 0.618506 |
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Target: 5'- -gUGCGAUgacUCGGAACCcgugCUCGGCg-- -3' miRNA: 3'- caACGCUA---GGUCUUGGa---GAGCCGaga -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 44973 | 0.68 | 0.584778 |
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Target: 5'- --aGCGGUUCGGGAUCUUgUCGGcCUCUu -3' miRNA: 3'- caaCGCUAGGUCUUGGAG-AGCC-GAGA- -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 41080 | 0.69 | 0.540427 |
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Target: 5'- cGUUGCGggCCAGGccggugACCUCgCGGC-Cg -3' miRNA: 3'- -CAACGCuaGGUCU------UGGAGaGCCGaGa -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 35230 | 0.7 | 0.507918 |
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Target: 5'- --aGUGGUCCgAGGGCUUCUCGGUcCa -3' miRNA: 3'- caaCGCUAGG-UCUUGGAGAGCCGaGa -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 14909 | 0.71 | 0.416115 |
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Target: 5'- --gGCGaAUCCAGGGCCUgaUCaGGCUCg -3' miRNA: 3'- caaCGC-UAGGUCUUGGAg-AG-CCGAGa -5' |
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| 7341 | 3' | -54 | NC_001900.1 | + | 34220 | 1.07 | 0.001489 |
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Target: 5'- gGUUGCGAUCCAGAACCUCUCGGCUCUu -3' miRNA: 3'- -CAACGCUAGGUCUUGGAGAGCCGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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