Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7342 | 3' | -56.1 | NC_001900.1 | + | 38603 | 0.66 | 0.645191 |
Target: 5'- cUCGuCCCAGcggcGCAUCuccucgCGCUcgGCGAu -3' miRNA: 3'- -GGC-GGGUCa---CGUAGua----GCGGuaCGCU- -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 30288 | 0.66 | 0.634157 |
Target: 5'- gCCGCCUucacgAGcGgGUCAUCGCCcgcUGCGc -3' miRNA: 3'- -GGCGGG-----UCaCgUAGUAGCGGu--ACGCu -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 27118 | 0.67 | 0.579153 |
Target: 5'- aCGCCCAGccucacggGC-UCAUCGCgAUGgUGAu -3' miRNA: 3'- gGCGGGUCa-------CGuAGUAGCGgUAC-GCU- -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 26015 | 0.67 | 0.556316 |
Target: 5'- aCCGCCCGGaguacguUGUAggCGUgcCGCCGUGCc- -3' miRNA: 3'- -GGCGGGUC-------ACGUa-GUA--GCGGUACGcu -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 31820 | 0.67 | 0.546607 |
Target: 5'- -aGCCCGGUGaagaacUCGCCAgUGCGGu -3' miRNA: 3'- ggCGGGUCACguagu-AGCGGU-ACGCU- -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 34072 | 0.67 | 0.544456 |
Target: 5'- aCCGCagCAGUcaaaacuGCAUCGUCguuuucuGCCAUGUGGc -3' miRNA: 3'- -GGCGg-GUCA-------CGUAGUAG-------CGGUACGCU- -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 25389 | 0.69 | 0.473324 |
Target: 5'- gCUGCUgAGgccaucugGUAcuUCAUCGCCAUGCGc -3' miRNA: 3'- -GGCGGgUCa-------CGU--AGUAGCGGUACGCu -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 36266 | 0.72 | 0.327496 |
Target: 5'- -aGCCCAGUuccuUCAUCGCCA-GCGGc -3' miRNA: 3'- ggCGGGUCAcgu-AGUAGCGGUaCGCU- -5' |
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7342 | 3' | -56.1 | NC_001900.1 | + | 34672 | 1.11 | 0.000556 |
Target: 5'- uCCGCCCAGUGCAUCAUCGCCAUGCGAg -3' miRNA: 3'- -GGCGGGUCACGUAGUAGCGGUACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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