Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7343 | 5' | -54.4 | NC_001900.1 | + | 19375 | 0.66 | 0.740898 |
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Target: 5'- -aAG-CAG-GUCGUGGacgGGUUCGACAa -3' miRNA: 3'- ggUCaGUCuCAGCAUCg--CCGAGCUGU- -5' |
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| 7343 | 5' | -54.4 | NC_001900.1 | + | 21966 | 0.66 | 0.740898 |
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Target: 5'- -uGGUCAGAGcacUUGUGgaaguGCGGCuuUCGGCAu -3' miRNA: 3'- ggUCAGUCUC---AGCAU-----CGCCG--AGCUGU- -5' |
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| 7343 | 5' | -54.4 | NC_001900.1 | + | 44148 | 0.66 | 0.740898 |
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Target: 5'- uCCAcuucGUCAG-GUCGUAG-GGCUUGGuCGg -3' miRNA: 3'- -GGU----CAGUCuCAGCAUCgCCGAGCU-GU- -5' |
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| 7343 | 5' | -54.4 | NC_001900.1 | + | 34523 | 0.66 | 0.734549 |
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Target: 5'- cCCGGUguGccuucguggagcgcgGGUUG-AGCGGgUCGACAg -3' miRNA: 3'- -GGUCAguC---------------UCAGCaUCGCCgAGCUGU- -5' |
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| 7343 | 5' | -54.4 | NC_001900.1 | + | 21593 | 0.69 | 0.565559 |
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Target: 5'- gCCGGcUCAaGGcCGU-GCGGCUCGGCc -3' miRNA: 3'- -GGUC-AGUcUCaGCAuCGCCGAGCUGu -5' |
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| 7343 | 5' | -54.4 | NC_001900.1 | + | 37476 | 0.69 | 0.554668 |
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Target: 5'- -aGGUCuucGGAGUCGguuccAGCGGCUUGuCAg -3' miRNA: 3'- ggUCAG---UCUCAGCa----UCGCCGAGCuGU- -5' |
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| 7343 | 5' | -54.4 | NC_001900.1 | + | 46004 | 0.69 | 0.54384 |
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Target: 5'- gCCGGUCGG-GUCGgcgGGUGcccGCUCGAUc -3' miRNA: 3'- -GGUCAGUCuCAGCa--UCGC---CGAGCUGu -5' |
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| 7343 | 5' | -54.4 | NC_001900.1 | + | 35041 | 1.11 | 0.000862 |
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Target: 5'- cCCAGUCAGAGUCGUAGCGGCUCGACAa -3' miRNA: 3'- -GGUCAGUCUCAGCAUCGCCGAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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