Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7346 | 5' | -53.5 | NC_001900.1 | + | 47337 | 0.66 | 0.784401 |
Target: 5'- cACUGg--GCAGACCGAugCUGUUCg- -3' miRNA: 3'- -UGGCaguUGUCUGGUUugGGCGAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 42064 | 0.66 | 0.774181 |
Target: 5'- cGCCGUCGuuguACGGGCCGgugacgugguggAGCCCGaUCa- -3' miRNA: 3'- -UGGCAGU----UGUCUGGU------------UUGGGCgAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 27076 | 0.66 | 0.77315 |
Target: 5'- aGCCaGUCGACAuGCCGAaaGCCgcacuuccacaagUGCUCUGa -3' miRNA: 3'- -UGG-CAGUUGUcUGGUU--UGG-------------GCGAGAC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 32464 | 0.66 | 0.763806 |
Target: 5'- -aCGUCGGCcGACagcuCCCGCUCg- -3' miRNA: 3'- ugGCAGUUGuCUGguuuGGGCGAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 36468 | 0.66 | 0.762761 |
Target: 5'- uGCCGUCGuucaccaGCAGGCUcAGgCCGUUCa- -3' miRNA: 3'- -UGGCAGU-------UGUCUGGuUUgGGCGAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 10492 | 0.66 | 0.753291 |
Target: 5'- gGCCG-CGAUGGGCCucGGCCUGCUg-- -3' miRNA: 3'- -UGGCaGUUGUCUGGu-UUGGGCGAgac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 48750 | 0.66 | 0.753291 |
Target: 5'- cACCGUCGucGCAGGuCCAuAGgCUGCUCa- -3' miRNA: 3'- -UGGCAGU--UGUCU-GGU-UUgGGCGAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 4259 | 0.66 | 0.752231 |
Target: 5'- cGCCGUgAcGCAGAUCAAGCUuccgcugCGCUCg- -3' miRNA: 3'- -UGGCAgU-UGUCUGGUUUGG-------GCGAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 35311 | 0.66 | 0.742645 |
Target: 5'- cGCCaGUCGuggacgcaguaGCAGACCGgagGAUCCGCUUcgUGg -3' miRNA: 3'- -UGG-CAGU-----------UGUCUGGU---UUGGGCGAG--AC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 25273 | 0.66 | 0.741573 |
Target: 5'- cCCGUCGuCGGACCAcagggccagucgaGAgCCGUUCUu -3' miRNA: 3'- uGGCAGUuGUCUGGU-------------UUgGGCGAGAc -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 36215 | 0.67 | 0.721014 |
Target: 5'- cGCCGUCGuCGuACCGAACCCugaucgcCUCUGc -3' miRNA: 3'- -UGGCAGUuGUcUGGUUUGGGc------GAGAC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 17018 | 0.67 | 0.721014 |
Target: 5'- gGCgG-CAGCcgAGACCAugauGCCCGCgCUGg -3' miRNA: 3'- -UGgCaGUUG--UCUGGUu---UGGGCGaGAC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 11741 | 0.67 | 0.721013 |
Target: 5'- cGCC--CAGCGGAUCAAGCCCGaUUCg- -3' miRNA: 3'- -UGGcaGUUGUCUGGUUUGGGC-GAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 6489 | 0.67 | 0.699013 |
Target: 5'- cGCUGUCAACGGcgcggucccccaGCUcgGCCCGCUg-- -3' miRNA: 3'- -UGGCAGUUGUC------------UGGuuUGGGCGAgac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 20718 | 0.67 | 0.699012 |
Target: 5'- cGCUGUCGAcCAGACCGAcggcaGCaaGCUgUGg -3' miRNA: 3'- -UGGCAGUU-GUCUGGUU-----UGggCGAgAC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 40840 | 0.67 | 0.699012 |
Target: 5'- uGCCuguGUCGACGGACUu--CCCGgaCUGc -3' miRNA: 3'- -UGG---CAGUUGUCUGGuuuGGGCgaGAC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 12896 | 0.68 | 0.665529 |
Target: 5'- cACCGUCGAgAGcCCGAACUucgucuCGCUgUGg -3' miRNA: 3'- -UGGCAGUUgUCuGGUUUGG------GCGAgAC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 43783 | 0.68 | 0.643023 |
Target: 5'- gGCCGgCAGCAGugCua--CCGCUCg- -3' miRNA: 3'- -UGGCaGUUGUCugGuuugGGCGAGac -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 23885 | 0.68 | 0.643023 |
Target: 5'- cAUCGUCGGCAGGCuCGccucguacccGGCCCGagcCUCUGc -3' miRNA: 3'- -UGGCAGUUGUCUG-GU----------UUGGGC---GAGAC- -5' |
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7346 | 5' | -53.5 | NC_001900.1 | + | 16366 | 0.68 | 0.631749 |
Target: 5'- aACCGUCAcgucgGCAGA-CGAACUCGCggaguucgCUGg -3' miRNA: 3'- -UGGCAGU-----UGUCUgGUUUGGGCGa-------GAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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