Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7347 | 5' | -55.3 | NC_001900.1 | + | 2013 | 0.66 | 0.661182 |
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Target: 5'- cGUGgGCGcGUGUCAuCCGAgaCUUCaGCGa -3' miRNA: 3'- -CAUgCGC-CACAGUcGGCU--GAAGaCGC- -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 7745 | 0.68 | 0.539961 |
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Target: 5'- -cGCGCuGUGUCucGCCGAacUCUGUGg -3' miRNA: 3'- caUGCGcCACAGu-CGGCUgaAGACGC- -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 9453 | 0.69 | 0.518546 |
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Target: 5'- cGUGUGCGGUG-CuGUCGuACUUCUGCc -3' miRNA: 3'- -CAUGCGCCACaGuCGGC-UGAAGACGc -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 15441 | 0.67 | 0.5836 |
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Target: 5'- -gGCGUGGaUGUCGccGCCGA--UCUGCa -3' miRNA: 3'- caUGCGCC-ACAGU--CGGCUgaAGACGc -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 31677 | 0.71 | 0.397854 |
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Target: 5'- --uCGCGGUGUCgAGCUGACUccgcagaUCgGCGu -3' miRNA: 3'- cauGCGCCACAG-UCGGCUGA-------AGaCGC- -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 33102 | 0.67 | 0.616789 |
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Target: 5'- -aGCGCGGcGaUCAGCUucuugGGCUUCgugGCGa -3' miRNA: 3'- caUGCGCCaC-AGUCGG-----CUGAAGa--CGC- -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 33401 | 0.67 | 0.616789 |
|
Target: 5'- ---aGCGGUGUCGaCCGGCgcgCUGgCGa -3' miRNA: 3'- caugCGCCACAGUcGGCUGaa-GAC-GC- -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 37038 | 1.08 | 0.001012 |
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Target: 5'- uGUACGCGGUGUCAGCCGACUUCUGCGu -3' miRNA: 3'- -CAUGCGCCACAGUCGGCUGAAGACGC- -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 39897 | 0.71 | 0.398777 |
|
Target: 5'- cGUGCagcaGCGGUGUCGuGUCGGCUUCaucaGCGc -3' miRNA: 3'- -CAUG----CGCCACAGU-CGGCUGAAGa---CGC- -5' |
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| 7347 | 5' | -55.3 | NC_001900.1 | + | 40801 | 0.72 | 0.35435 |
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Target: 5'- --uCGgGGUGUCGGCCGgcGCUUCgagaGCGu -3' miRNA: 3'- cauGCgCCACAGUCGGC--UGAAGa---CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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