miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
735 5' -60.4 AC_000020.1 + 595 1.09 0.000121
Target:  5'- uUCUCAUGGCAGCCCCUCGCCGCCUACu -3'
miRNA:   3'- -AGAGUACCGUCGGGGAGCGGCGGAUG- -5'
735 5' -60.4 AC_000020.1 + 27268 0.66 0.270112
Target:  5'- cUUUgGUGGCAaCCCCUCccCCGCCUc- -3'
miRNA:   3'- -AGAgUACCGUcGGGGAGc-GGCGGAug -5'
735 5' -60.4 AC_000020.1 + 14365 0.68 0.206016
Target:  5'- aCUCuccGGCAGCCUCcagcaccaagcgCGCCGCCg-- -3'
miRNA:   3'- aGAGua-CCGUCGGGGa-----------GCGGCGGaug -5'
735 5' -60.4 AC_000020.1 + 14731 0.7 0.136619
Target:  5'- gCUCAuccuUGGCAGCCCUgCGCUuUCUGCu -3'
miRNA:   3'- aGAGU----ACCGUCGGGGaGCGGcGGAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.