Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7350 | 3' | -57.9 | NC_001900.1 | + | 41237 | 0.66 | 0.518488 |
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Target: 5'- gGCCUUgcgggGCAuGGCGUUCGgaGUGGUCa -3' miRNA: 3'- aCGGGGg----UGU-CCGCGAGCaaCAUCAG- -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 35197 | 0.67 | 0.45697 |
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Target: 5'- cGCCCuCCAUgAGGCGCaugaacgugaauUCGaaGUGGUCc -3' miRNA: 3'- aCGGG-GGUG-UCCGCG------------AGCaaCAUCAG- -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 21291 | 0.67 | 0.437341 |
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Target: 5'- cUGCCCCCGCuGGGCGgUCuGaUGgaugcGGUCc -3' miRNA: 3'- -ACGGGGGUG-UCCGCgAG-CaACa----UCAG- -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 3756 | 0.67 | 0.437341 |
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Target: 5'- gUGaCCUCGC-GGCGCUCGUucgaUGaGGUCg -3' miRNA: 3'- -ACgGGGGUGuCCGCGAGCA----ACaUCAG- -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 28377 | 0.69 | 0.363983 |
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Target: 5'- cUGCCaCUGCAGGUGCUCGUcaccGUCg -3' miRNA: 3'- -ACGGgGGUGUCCGCGAGCAacauCAG- -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 36240 | 0.69 | 0.355431 |
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Target: 5'- cGCCUCUGCGuGGCGCUCGUUcuUGGa- -3' miRNA: 3'- aCGGGGGUGU-CCGCGAGCAAc-AUCag -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 9764 | 0.7 | 0.292193 |
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Target: 5'- cGaCCCCCACGGGCucgGCgCGgucccgGUGGUCc -3' miRNA: 3'- aC-GGGGGUGUCCG---CGaGCaa----CAUCAG- -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 27816 | 0.89 | 0.011989 |
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Target: 5'- aGCUCCCGCAGGCGCUCcGUUGUAGcCa -3' miRNA: 3'- aCGGGGGUGUCCGCGAG-CAACAUCaG- -5' |
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| 7350 | 3' | -57.9 | NC_001900.1 | + | 37586 | 1.1 | 0.000352 |
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Target: 5'- gUGCCCCCACAGGCGCUCGUUGUAGUCg -3' miRNA: 3'- -ACGGGGGUGUCCGCGAGCAACAUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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